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Commit 1ecf02a3 authored by Venkat Malladi's avatar Venkat Malladi
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Add reference snippet. Close #85.

parent 281fb224
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2 merge requests!58Develop,!57Prep of 1.0.0 release
# v0.1.0 (in development)
# v1.0.0 (in development)
**User Facing**
* Add option to pull references from datahub
* Add option to send email on workflow error, with pipeline error message
......@@ -17,6 +17,7 @@
* Error if pe and line count of R1 != R2
* Error if ambiguous species inference
* Remove non fastq from inputBag from the export bag config level
* Add link to reference builder script
**Background**
* Remove (comment out) option to pull references from S3
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......@@ -75,6 +75,11 @@ FULL EXAMPLE:
nextflow run workflow/rna-seq.nf --repRID Q-Y5JA --source production --deriva ./data/credential.json --bdbag ./data/cookies.txt --dev false --upload true -profile biohpc
```
To generate you own references or new references:
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Download the [reference creation script](https://git.biohpc.swmed.edu/gudmap_rbk/rna-seq/-/snippets/31).
This script will auto create human and mouse references from GENCODE. It can also create ERCC92 spike-in references as well as concatenate them to GENCODE references automatically. In addition, it can create references from manually downloaded FASTA and GTF files.
To run a set of replicates from study RID:
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Run in repo root dir:
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