From 1ecf02a3117b6c5af448171d18d6131ad7ed4fca Mon Sep 17 00:00:00 2001 From: Venkat Malladi <venkat.malladi@utsouthwestern.edu> Date: Fri, 8 Jan 2021 14:11:22 -0600 Subject: [PATCH] Add reference snippet. Close #85. --- CHANGELOG.md | 3 ++- README.md | 5 +++++ 2 files changed, 7 insertions(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 6c77f50..30d202a 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -1,4 +1,4 @@ -# v0.1.0 (in development) +# v1.0.0 (in development) **User Facing** * Add option to pull references from datahub * Add option to send email on workflow error, with pipeline error message @@ -17,6 +17,7 @@ * Error if pe and line count of R1 != R2 * Error if ambiguous species inference * Remove non fastq from inputBag from the export bag config level +* Add link to reference builder script **Background** * Remove (comment out) option to pull references from S3 diff --git a/README.md b/README.md index 65e4e73..b21653d 100644 --- a/README.md +++ b/README.md @@ -75,6 +75,11 @@ FULL EXAMPLE: nextflow run workflow/rna-seq.nf --repRID Q-Y5JA --source production --deriva ./data/credential.json --bdbag ./data/cookies.txt --dev false --upload true -profile biohpc ``` +To generate you own references or new references: +------------------------------------------ +Download the [reference creation script](https://git.biohpc.swmed.edu/gudmap_rbk/rna-seq/-/snippets/31). +This script will auto create human and mouse references from GENCODE. It can also create ERCC92 spike-in references as well as concatenate them to GENCODE references automatically. In addition, it can create references from manually downloaded FASTA and GTF files. + To run a set of replicates from study RID: ------------------------------------------ Run in repo root dir: -- GitLab