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GUDMAP_RBK
RNA-seq
Commits
0bb07de4
Commit
0bb07de4
authored
4 years ago
by
Gervaise Henry
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Move spike/stranded override to infer (like species), not submitter
parent
f36d728b
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2 merge requests
!68
Develop
,
!67
Resolve "Change Single Read to Single End for submitted endness"
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#9172
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in 4 hours, 8 minutes, and 49 seconds
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workflow/rna-seq.nf
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0bb07de4
...
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@@ -320,8 +320,6 @@ process parseMetadata {
path experiment from experimentMeta
path (fastq) from fastqs_parseMetadata.collect()
val fastqCount
val strandedForce
val spikeForce
output:
path "design.csv" into metadata_fl
...
...
@@ -379,11 +377,6 @@ process parseMetadata {
then
stranded="_No value_"
fi
if [ "${strandedForce}" != "" ]
then
stranded=${strandedForce}
echo -e "LOG: spike-in metadata forced: \${stranded}" >> ${repRID}.parseMetadata.log
fi
# get spike-in metadata
spike=\$(python3 ${script_parseMeta} -r ${repRID} -m "${experimentSettings}" -p spike)
...
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@@ -392,11 +385,6 @@ process parseMetadata {
then
spike="_No value_"
fi
if [ "${spikeForce}" != "" ]
then
spike=${spikeForce}
echo -e "LOG: spike-in metadata forced: \${spike}" >> ${repRID}.parseMetadata.log
fi
if [ "\${spike}" == "f" ]
then
spike="false"
...
...
@@ -952,6 +940,8 @@ process inferMetadata {
path bam from sampleBam.collect()
path bai from sampleBai.collect()
path alignSummary from alignSampleQC_inferMetadata.collect()
val strandedForce
val spikeForce
val fastqCountError_inferMetadata
val fastqReadError_inferMetadata
val fastqFileError_inferMetadata
...
...
@@ -990,6 +980,11 @@ process inferMetadata {
spike="false"
fi
echo -e "LOG: inference of strandedness results is: \${spike}" >> ${repRID}.inferMetadata.log
if [ "${spikeForce}" != "" ]
then
spike=${spikeForce}
echo -e "LOG: spike-in metadata forced: \${spike}" >> ${repRID}.parseMetadata.log
fi
speciesError=false
speciesError_details=""
...
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@@ -1062,6 +1057,11 @@ process inferMetadata {
stranded="unstranded"
fi
echo -e "LOG: stradedness set to: \${stranded}" >> ${repRID}.inferMetadata.log
if [ "${strandedForce}" != "" ]
then
stranded=${strandedForce}
echo -e "LOG: spike-in metadata forced: \${stranded}" >> ${repRID}.inferMetadata.log
fi
else
ends=""
stranded=""
...
...
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