multiqc_config.yaml

custom_logo: './bicf_logo.png'
custom_logo_url: 'https/utsouthwestern.edu/labs/bioinformatics/'
custom_logo_title: 'Bioinformatics Core Facility'

report_header_info:
  - Contact Email: 'bicf@utsouthwestern.edu'
  - Application Type: 'RNA-Seq Analytic Pipeline for GUDMAP/RBK'
  - Department: 'Bioinformatic Core Facility, Department of Bioinformatics, University of Texas Southwestern Medical Center'

title: RNA-Seq Analytic Pipeline for GUDMAP/RBK

report_comment: >
  This report has been generated by the <a href="https://doi.org/10.5281/zenodo.3625056">GUDMAP/RBK RNA-Seq Pipeline</a>

top_modules:
  - fastqc:
      name: 'Raw'
      info: 'Replicate Raw fastq QC Results'
  - cutadapt:
      name: 'Trim'
      info: 'Replicate Trim Adapter QC Results'
  - hisat2:
      name: 'Align'
      info: 'Replicate Alignment QC Results'
      path_filters:
        - '*alignSummary*'
  - picard:
      name: 'Dedup'
      info: 'Replicate Alignement Deduplication QC Results'
  - rseqc:
      name: 'Inner Distance'
      info: 'Replicate Paired End Inner Distance Distribution Results'
      path_filters:
        - '*insertSize*'
  - custom_content
  - featureCounts:
      name: 'Count'
      info: 'Replicate Feature Count QC Results'
  - hisat2:
      name: 'Inference: Align'
      info: 'Inference Alignment (1M downsampled reads) QC Results'
      path_filters:
        - '*alignSampleSummary*'
  - rseqc:
      name: 'Inference: Stranded'
      info: '1M Downsampled Reads Strandedness Inference Results'
      path_filters:
        - '*infer_experiment*'

report_section_order:
    rid:
      order: 2000
    meta:
      order: 1000

skip_generalstats: true

custom_data:
    rid:
        file_format: 'tsv'
        section_name: 'RID'
        description: 'This is the identifying RIDs'
        plot_type: 'table'
        pconfig:
            id: 'rid'
        headers:
            Replicate RID
            Experiment RID
            Study RID
    meta:
        file_format: 'tsv'
        section_name: 'Metadata'
        description: 'This is the comparison of infered metadata, submitter provided, and calculated'
        plot_type: 'table'
        pconfig:
            id: 'meta'
            format: '{:,.0f}'
        headers:
            Source
            Species
            Ends
            Stranded
            Spike-in
            Raw Reads
            Assigned Reads
            Median Read Length
            Median TIN
    tin:
        file_format: 'tsv'
        section_name: 'TIN'
        description: 'This is the distribution of TIN values calculated by the tool RSeQC'
        plot_type: 'bargraph'
        pconfig:
            id: 'tin'
        headers:
            chrom
            0 - 9
            10 - 19
            20 - 29
            30 - 39
            40 - 49
            50 - 59
            60 - 69
            70 - 79
            80 - 89
            90 - 99

sp:
    rid:
        fn: 'rid.tsv'
    meta:
        fn: 'metadata.tsv'
    tin:
        fn: '*.tin.hist.tsv'