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NGS CLIA Lab
process_scripts
Commits
0ccf418d
Commit
0ccf418d
authored
7 years ago
by
Brandi Cantarel
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typo markdups
parent
3d9955f6
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publish_0.0.10
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alignment/markdups.sh
+2
-2
2 additions, 2 deletions
alignment/markdups.sh
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2 additions
and
2 deletions
alignment/markdups.sh
+
2
−
2
View file @
0ccf418d
...
...
@@ -48,7 +48,7 @@ then
samtools markdup
-s
--output-fmt
BAM -@
$SLURM_CPUS_ON_NODE
sort.bam
${
pair_id
}
.dedup.bam
elif
[
$algo
==
'picard'
]
then
java
-Djava
.io.tmpdir
=
./
-Xmx4g
-jar
$PICARD
/picard.jar MarkDuplicates
I
=
${
sbam
}
O
=
${
p
refix
}
.dedup.bam
M
=
${
pair_id
}
.dedup.stat.txt
java
-Djava
.io.tmpdir
=
./
-Xmx4g
-jar
$PICARD
/picard.jar MarkDuplicates
I
=
${
sbam
}
O
=
${
p
air_id
}
.dedup.bam
M
=
${
pair_id
}
.dedup.stat.txt
elif
[
$algo
==
'picard_umi'
]
then
java
-Djava
.io.tmpdir
=
./
-Xmx4g
-jar
$PICARD
/picard.jar MarkDuplicates
BARCODE_TAG
=
RX
I
=
${
sbam
}
O
=
${
pair_id
}
.dedup.bam
M
=
${
pair_id
}
.dedup.stat.txt
...
...
@@ -67,5 +67,5 @@ then
bwa mem
-M
-C
-t
2
-R
'@RG\tID:${pair_id}\tLB:tx\tPL:illumina\tPU:barcode\tSM:${pair_id}'
/project/shared/bicf_workflow_ref/GRCh38/genome.fa
${
pair_id
}
.consensus.R1.fastq.gz
${
pair_id
}
.consensus.R2.fastq.gz | samtools view
-1
-
>
${
pair_id
}
.consensus.bam
samtools
sort
--threads
10
-o
${
pair_id
}
.dedup.bam
${
pair_id
}
.consensus.bam
else
cp
${
sbam
}
${
p
refix
}
.dedup.bam
cp
${
sbam
}
${
p
air_id
}
.dedup.bam
fi
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