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Commit c2b05eaa authored by Jonathan Gesell's avatar Jonathan Gesell
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Updated for concise output files

parent 9c845c6e
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3 merge requests!37v0.0.1,!4Develop,!2Resolve "process_getData"
#!/usr/bin/env nextflow
// Define input variables
params.deriva = "${baseDir}/../test_data/deriva-cookies.txt"
params.deriva = "/project/BICF/BICF_Core/shared/gudmap/cookies/deriva-cookies.txt"
params.bdbag = "${baseDir}/../test_data/Study_Q-Y4H0.zip"
params.outDir = "${baseDir}/../output"
// Parse input variables
deriva = file(params.deriva)
deriva = file(params.deriva, checkIfExists: 'true')
bdbag = Channel
.fromPath(params.bdbag)
.ifEmpty { exit 1, "bdbag zip file not found: ${params.bdbag}" }
......@@ -19,10 +19,10 @@ outDir = params.outDir
*/
process splitData {
tag "${bdbag.baseName}"
publishDir "${outDir}/temp/${task.process}", mode: "symlink"
input:
file bdbag
path cookies, stageAs: 'cookies.txt' from deriva
output:
file("Replicate_*.zip") into bdbagSplit mode flatten
......@@ -34,48 +34,44 @@ process splitData {
"""
hostname
ulimit -a
ln -sf `readlink -e ${deriva}` ~/.bdbag/deriva-cookies.txt
echo LOG: deriva cookie linked
study=`echo "${bdbag}" | cut -d'.' -f1`
echo LOG: \${study}
ln -sf `readlink -e cookies.txt` ~/.bdbag/deriva-cookies.txt
echo "LOG: deriva cookie linked"
study=`echo "${bdbag}" | cut -d '.' -f1`
echo "LOG: \${study}"
unzip ${bdbag}
echo LOG: bdgag unzipped
echo "LOG: bdgag unzipped"
python3 ${baseDir}/scripts/modifyFetch.py --fetchFile \${study}
echo LOG: fetch file filtered for only .fastq.gz
echo "LOG: fetch file filtered for only .fastq.gz"
python3 ${baseDir}/scripts/splitFetch.py --fetchFile \${study}
echo LOG: fetch file split by replicates
echo "LOG: fetch file split by replicates"
sh ${baseDir}/scripts/splitBag.sh \${study}
echo LOG: bag recreated with replicate split fetch file
echo "LOG: bag recreated with replicate split fetch file"
"""
}
println {${http_proxy}}
println {${https_proxy}}
/*
* getData: fetch study files from consortium with downloaded bdbag.zip
*/
process getData {
tag "${rep.baseName}"
publishDir "${outDir}/temp/${task.process}", mode: "symlink"
publishDir "${outDir}/tempOut/fastqs", mode: "symlink"
input:
each rep from bdbagSplit
output:
file("**/*.R*.fastq.gz") into fastq
path ("*.R*.fastq.gz", type: 'file', maxDepth: '0') into fastq
script:
"""
hostname
ulimit -a
echo LOG:\${http_proxy}
export https_proxy=\${http_proxy}
replicate=\$(echo "${rep}" | cut -d'.' -f1 | rev | cut -f1 -d '/' | rev)
echo LOG: \${replicate}
replicate=\$(basename "${rep}" | cut -d '.' -f1)
echo "LOG: \${replicate}"
unzip ${rep}
echo LOG: replicate bdbag unzipped
echo "LOG: replicate bdbag unzipped"
sh ${baseDir}/scripts/bdbagFetch.sh \${replicate}
echo LOG: replicate bdbag fetched
echo "LOG: replicate bdbag fetched"
"""
}
#!/bin
#!/bin/bash
bdbag --resolve-fetch all --fetch-filter filename\$*fastq.gz $1
\ No newline at end of file
bdbag --resolve-fetch all --fetch-filter filename\$*fastq.gz $1 &&
for i in $(find */ -name "*.R*.fastq.gz"); do
mv ${i} .;
done;
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