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GUDMAP_RBK
RNA-seq
Commits
445f6693
Commit
445f6693
authored
3 years ago
by
Gervaise Henry
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Change seqwho ref location
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da9f1020
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!95
Update rna-seq.nf
,
!94
Resolve "Move seqwho reference"
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#10528
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rna-seq.nf
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445f6693
# v.2.0.1 (indev)
**User Facing**
*
Corrected spelling of inferred (#125)
*
Add param for seqwho reference (#127)
**Background**
*
Corrected file search parameters due to name inconsistency (#129)
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@@ -11,6 +12,7 @@
*
Remove "fail" integration CI tests (#133 addendum)
*
Add licence header to nf file, R and python scriptsand pytest scripts
*
Stop using RCB and RMB (default) with deriva.core insert calls to match server update (#134)
*
Replace default seqwho reference location to one provided by source lab (#127)
# v2.0.0
**User Facing**
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rna-seq.nf
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@@ -23,6 +23,7 @@ params.source = "dev"
params.refMoVersion = "38.p6.vM25"
params.refHuVersion = "38.p13.v36"
params.refERCCVersion = "92"
params.seqwhoRef = "https://cloud.biohpc.swmed.edu/index.php/s/sP48taKmymSkJBM/download"
params.outDir = "${baseDir}/output"
params.upload = false
params.email = ""
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@@ -67,6 +68,7 @@ repRID = params.repRID
refMoVersion = params.refMoVersion
refHuVersion = params.refHuVersion
refERCCVersion = params.refERCCVersion
seqwhoRef = params.seqwhoRef
outDir = params.outDir
logsDir = "${outDir}/Logs"
upload = params.upload
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@@ -714,6 +716,7 @@ process seqwho {
tag "${repRID}"
input:
val seqwhoRef
path (fastq) from fastqs_seqwho
val ends from endsManual_seqwho
val speciesMeta from speciesMeta_seqwho
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@@ -734,7 +737,7 @@ process seqwho {
ulimit -a >> ${repRID}.seqwho.log
# get seqwho index
wget -O SeqWho.ix
https://cloud.biohpc.swmed.edu/index.php/s/eeNWqZz8jqN5zWY/download
wget -O SeqWho.ix
${seqwhoRef}
echo -e "LOG: seqwho index downloaded" >> ${repRID}.seqwho.log
# run seqwho
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