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CHANGELOG.md 6.93 KiB

v2.0.0

User Facing

  • Endness metadata "Single Read" changed to "Single End" in data-hub, pipeline updated to handle (#110) ("Single Read" still acceptable for backwards compatibility)
  • Strandedness metadata "yes"/"no" changed to boolean "t"/"f" in data-hub, pipeline updated to handle (#70) ("yes"/"no" still acceptable for backwards compatibility)
  • Upload empty mRNA_QC entry if data error (#111)
  • Allow forcing of strandedness and spike (#100)

Background

  • Add memory limit (75%) per thread for samtools sort (#108)
  • Remove parsing restrictions for submitted stranded/spike/species (#105, #106)
  • Pass unidentified ends instead of overwriting it as unknown
  • Move fastqc process before trim to catch fastq errors (#107)
  • Only use fastq's that match *[_.]R[1-2].fastq.gz naming convention (#107)
  • Add error output for no fastq's
  • Update input bag export config to only fetch fastq's that match *[_.]R[1-2].fastq.gz naming convention
  • Remove check for multiple fastq check in parse metadata (redundant and no longer valid)
  • Handle blank submitted endness better
  • Don't use file.csv from inputBag to parse manual endness, use counted from getData
  • Detect malformed fastq's (#107)
  • Restrict sampled alignment process to use >32GB nodes on BioHPC (#108)
  • Use nproc**-1** for alignment processes (#108)
  • Data-hub column title change from "Sequencing_Type" to "Experiment_Type" (#114)
  • Data-hub column title change from "Has_Strand_Specific_Information" to "Strandedness" (#115)
  • Merge data error pre-inference execution run upload/finalize to 1 process
  • Change uploadOutputBag logic to change reuse hatrac file if alread exists (re-uses Output_Bag entry by reassigning Execution_Run RID) (#112)
  • Add new CI py tests for override and integration

Known Bugs

  • Override params (inputBag, fastq, species) aren't checked for integrity
  • Authentication files and tokens must be active (active auth client) for the duration of the pipeline run (until long-lived token utilization included)
  • Check for outputBag in hatrac doesn't check for any uploaded by chaise

v1.0.2

User Facing

Background

  • Fix spelling in config file for process of failed fastq to upload error message (#104)

Known Bugs

  • Override params (inputBag, fastq, species) aren't checked for integrity
  • Authentication files and tokens must be active (active auth client) for the duration of the pipeline run (until long-lived token utilization included)

v1.0.1

User Facing

Background

  • Split non-metadata mismatch error handling proces into 2, 1 to handle fastq errors and one for species errors (BUG FIX #101)
  • Add known errors to integration CI tests (ambiguous species, trunkated fastq, R1/R2 mismatch (#103)
  • Fix pre exeuction run fails uploading of execution run RID to tracking site (#96, #97)
  • Change CI replicate count badge CI to count all execution runs that match major version

Known Bugs

  • Override params (inputBag, fastq, species) aren't checked for integrity
  • Authentication files and tokens must be active (active auth client) for the duration of the pipeline run (until long-lived token utilization included)

v1.0.0

User Facing

  • Add link to reference builder script
  • Output median TIN to mRNA_QC table

Background

  • Change consistency test to check if +/- 5% of standard
  • Change tool version checker for badges to use latest tag
  • Utilize pipeline tracking and qc AWS tables

Known Bugs

  • Override params (inputBag, fastq, species) aren't checked for integrity
  • Authentication files and tokens must be active (active auth client) for the duration of the pipeline run (until long-lived token utilization included)

v0.1.0

User Facing

  • Add option to pull references from datahub
  • Add option to send email on workflow error, with pipeline error message
  • Add versions and paper references of software used to report
  • Upload input bag
  • Upload execution run
  • Upload mRNA QC
  • Create and upload output bag
  • Add optional to not upload
  • Update references to use bags
  • Update to newer references (GRCh38.p13.v36 and GRCm38.p6.vM25)
  • Use production server for data-hub reference call
  • Error pipeline if submitted does not match infered
  • Update execution run with "Success" or "Error"
  • Error if fastq error (>2, if pe != 2, if se !=1)
  • Error if pe and line count of R1 != R2
  • Error if ambiguous species inference
  • Remove non fastq from inputBag from the export bag config level

Background

  • Remove (comment out) option to pull references from S3
  • Make pull references from BioHPC default (including in biohpc.config)
  • Start using new gudmaprbk dockerhub (images autobuilt)
  • Moved consistency checks to be fully python
  • Changed order of steps so that fastqc is done after the trim step
  • Change docker images to production
  • Add automated version badges
  • Only calculate/report tin values on regular chromosomes (from gtf)
  • Change inputBag fetch to manifest then validate (if fail fetch missing and revalidate up to 3 times)
  • Retry getData and trimData processes up to once
  • Make inputBag export config to create inputBag with only small txt file for CI unit test of getData (and update test)

Known Bugs

  • Override params (inputBag, fastq, species) aren't checked for integrity

v0.0.3

User Facing

  • TPM table:
    • Add Ensembl Gene ID
    • Rename columns: GENCODE_Gene_Symbol, Ensembl_GeneID, NCBI_GeneID
  • MultiQC output custom tables (html+JSON):
    • Run table: Session ID and Pipeline Version
    • Reference Table: Species, Genome Reference Consortium Build, Genome Reference Consortium Patch, GENCODE Annotation Release (outputs both human and mouse versions)
  • Add inputBag override param (inputBagForce) [*.zip]
    • Uses provided inputBag instead of downloading from data-hub
    • Still requires matching repRID input param
  • Add fastq override param (fastqsForce) [R1,R2]
    • Uses provided fastq instead of downloading from data-hub
    • Still requires matching repRID input param and will pull inputBag from data-hub to access submitted metadata for reporting
  • Add species override param (speciesForce) [Mus musculus or Homo sapiens]
    • forces the use of the provided species
    • ignores inferred ambiguous species

Background

  • Add GeneSymbol/EnsemblID/EntrezID translation files to references

Known Bugs

  • outputBag does not contain fetch for processed data
  • Does not include automatic data upload
  • Override params (inputBag, fastq, species) aren't checked for integrity

v0.0.2

User Facing

  • Output:
    • inputBag
    • outputBag
  • Remove gene details from tpm table
  • Add EntrezID translation to tpm table (from version specific reference)

Background

  • Add GeneSymbol/EnsemblID/EntrezID translation files to references

Known Bugs

  • outputBag does not contain fetch for processed data
  • Does not include automatic data upload

v0.0.1

INITIAL BETA VERSION
Does not include automatic data upload
This version is for initial upload of test data to GUDMAP/RBK data-hub for internal integration