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NGS CLIA Lab
process_scripts
Commits
e5a54f52
Commit
e5a54f52
authored
4 years ago
by
Brandi Cantarel
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update small docker/dnanexus incompatobility
parent
ba8a20f1
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2
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2 changed files
variants/germline_vc.sh
+3
-1
3 additions, 1 deletion
variants/germline_vc.sh
variants/somatic_vc.sh
+1
-0
1 addition, 0 deletions
variants/somatic_vc.sh
with
4 additions
and
1 deletion
variants/germline_vc.sh
+
3
−
1
View file @
e5a54f52
...
...
@@ -86,7 +86,9 @@ then
for
i
in
*
.bam
;
do
bamlist
=
"
$bamlist
--bam
${
PWD
}
/
${
i
}
"
done
cut
-f
1
$fbsplit
| parallel
--memfree
2G
--delay
1
--jobs
0
"freebayes -f
${
index_path
}
/genome.fa --min-mapping-quality 0 --min-base-quality 20 --min-coverage 10 --min-alternate-fraction 0.01 -C 3 --use-best-n-alleles 3 -r {}
${
bamlist
}
> fb.{}.vcf"
#--memfree 2G for DNANexus
cut
-f
1
$fbsplit
| parallel
--delay
1
--jobs
0
"freebayes -f
${
index_path
}
/genome.fa --min-mapping-quality 0 --min-base-quality 20 --min-coverage 10 --min-alternate-fraction 0.01 -C 3 --use-best-n-alleles 3 -r {}
${
bamlist
}
> fb.{}.vcf"
vcf-concat fb.
*
.vcf | vcf-sort | vcf-annotate
-n
--fill-type
| bcftools norm
-c
s
-f
${
reffa
}
-w
10
-O
z
-o
${
pair_id
}
.fb.vcf.gz -
elif
[[
$algo
==
'platypus'
]]
then
...
...
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variants/somatic_vc.sh
+
1
−
0
View file @
e5a54f52
...
...
@@ -142,6 +142,7 @@ then
elif
[
$algo
==
'shimmer'
]
then
module load R/3.6.1-gccmkl
module
rm
perl/5.18.2
shimmer.pl
--minqual
25
--ref
${
reffa
}
${
normal
}
${
tumor
}
--outdir
shimmer 2> shimmer.err
perl
$baseDir
/add_readct_shimmer.pl
module
rm
java/oracle/jdk1.7.0_51
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