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chipseq_analysis
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Astrocyte
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BICF
chipseq_analysis
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6cee390c
Commit
6cee390c
authored
8 years ago
by
Beibei Chen
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Merge branch 'develop' into 'master'
update R moudle See merge request
!2
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81b4c97b
77a40f95
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workflow/main.nf
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6cee390c
#!/usr/bin/env nextflow
#!/usr/bin/env nextflow
params.design="$baseDir/../test/samplesheet.csv"
params.design="$baseDir/../test
_data
/samplesheet.csv"
params.bams = "$baseDir/../test/*.bam"
params.bams = "$baseDir/../test
_data
/*.bam"
params.bais = "$baseDir/../test/*.bai"
params.bais = "$baseDir/../test
_data
/*.bai"
params.peaks = "$baseDir/../test/*.broadPeak"
params.peaks = "$baseDir/../test
_data
/*.broadPeak"
params.genomepath="/project/shared/bicf_workflow_ref/hg19/"
params.genomepath="/project/shared/bicf_workflow_ref/hg19/"
species = "hg19"
species = "hg19"
toppeakcount = 200
toppeakcount = 200
...
@@ -32,7 +32,7 @@
...
@@ -32,7 +32,7 @@
// script:
// script:
// """
// """
// module load python/2.7.x-anaconda
// module load python/2.7.x-anaconda
//
source activate /project/shared/bicf_workflow_ref/chipseq_bchen4/
//
module load R/3.3.2-gccmkl
// module load samtools/intel/1.3
// module load samtools/intel/1.3
// samtools index $index_bam_files
// samtools index $index_bam_files
// """
// """
...
@@ -50,7 +50,7 @@ process run_deeptools {
...
@@ -50,7 +50,7 @@ process run_deeptools {
script:
script:
"""
"""
module load python/2.7.x-anaconda
module load python/2.7.x-anaconda
source activate /project/shared/bicf_workflow_ref/chipseq_bchen4/
module load R/3.3.2-gccmkl
module load deeptools/2.3.5
module load deeptools/2.3.5
python $baseDir/scripts/runDeepTools.py -i ${params.design} -g ${params.genomepath}}
python $baseDir/scripts/runDeepTools.py -i ${params.design} -g ${params.genomepath}}
"""
"""
...
@@ -72,7 +72,7 @@ process run_diffbind {
...
@@ -72,7 +72,7 @@ process run_diffbind {
script:
script:
"""
"""
module load python/2.7.x-anaconda
module load python/2.7.x-anaconda
source activate /project/shared/bicf_workflow_ref/chipseq_bchen4/
module load R/3.3.2-gccmkl
Rscript $baseDir/scripts/runDiffBind.R $diffbind_design_file
Rscript $baseDir/scripts/runDiffBind.R $diffbind_design_file
"""
"""
}
}
...
@@ -87,7 +87,7 @@ process run_chipseeker_diffpeak {
...
@@ -87,7 +87,7 @@ process run_chipseeker_diffpeak {
script:
script:
"""
"""
module load python/2.7.x-anaconda
module load python/2.7.x-anaconda
source activate /project/shared/bicf_workflow_ref/chipseq_bchen4/
module load R/3.3.2-gccmkl
Rscript $baseDir/scripts/runChipseeker.R $diffpeak_design_file hg19
Rscript $baseDir/scripts/runChipseeker.R $diffpeak_design_file hg19
"""
"""
}
}
...
@@ -102,7 +102,7 @@ process run_chipseeker_originalpeak {
...
@@ -102,7 +102,7 @@ process run_chipseeker_originalpeak {
script:
script:
"""
"""
module load python/2.7.x-anaconda
module load python/2.7.x-anaconda
source activate /project/shared/bicf_workflow_ref/chipseq_bchen4/
module load R/3.3.2-gccmkl
Rscript $baseDir/scripts/runChipseeker.R $design_file ${species}
Rscript $baseDir/scripts/runChipseeker.R $design_file ${species}
"""
"""
}
}
...
@@ -117,7 +117,7 @@ process run_meme_original {
...
@@ -117,7 +117,7 @@ process run_meme_original {
script:
script:
"""
"""
module load python/2.7.x-anaconda
module load python/2.7.x-anaconda
source activate /project/shared/bicf_workflow_ref/chipseq_bchen4/
module load R/3.3.2-gccmkl
module load meme/4.11.1-gcc-openmpi
module load meme/4.11.1-gcc-openmpi
python $baseDir/scripts/runMemechip.py -i $design_meme -g ${params.genomepath} -l ${toppeakcount}
python $baseDir/scripts/runMemechip.py -i $design_meme -g ${params.genomepath} -l ${toppeakcount}
"""
"""
...
@@ -133,7 +133,7 @@ process run_meme_diffpeak {
...
@@ -133,7 +133,7 @@ process run_meme_diffpeak {
script:
script:
"""
"""
module load python/2.7.x-anaconda
module load python/2.7.x-anaconda
source activate /project/shared/bicf_workflow_ref/chipseq_bchen4/
module load R/3.3.2-gccmkl
module load meme/4.11.1-gcc-openmpi
module load meme/4.11.1-gcc-openmpi
python $baseDir/scripts/runMemechip.py -i $diffpeak_design -g ${params.genomepath} -l ${toppeakcount}
python $baseDir/scripts/runMemechip.py -i $diffpeak_design -g ${params.genomepath} -l ${toppeakcount}
"""
"""
...
...
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