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Commit 71085fe9 authored by Gervaise Henry's avatar Gervaise Henry 🤠
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Update README.md (Add lab info and update some formatting)

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Determining cellular heterogeneity in the human prostate with single-cell RNA sequencing
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* Contact: Gervaise H. Henry
* Email: gervaise.henry@utsouthwestern.edu
* Contact: **Gervaise H. Henry**
* Email: [gervaise.henry@utsouthwestern.edu](mailto:gervaise.henry@utsouthwestern.edu)
* Institution: UT Southwestern Medical Center
* Department: Urology
* Lab: Strand Lab
* PI: Douglas W. Strand, PhD
* PI Email: [douglas.strand@utsouthwestern.edu](mailto:douglas.strand@utsouthwestern.edu)
## Data Analysis
* Requirements:
* 10x CellRanger analyzed data:
* "filtered\_gene\_bc\_matrice_mex" folder
* csv file cellranger aggr used for aggregation
* demultiplex csv file to define subsets of samples
* **Requirements**:
* 10x CellRanger analyzed data:
* "filtered\_gene\_bc\_matrice_mex" folder
* csv file cellranger aggr used for aggregation
* demultiplex csv file to define subsets of samples
* Pipeline (r.scripts):
* **Pipeline** (r.scripts):
* sc_Demultiplex (import 10x Cell Ranger data into R and subset samples based on user input)
* sc_SeuratScore.CellCycle (identify cell cycle state)
* sc_QC (filter cells, scale and remove variation associated to: UMI; % mitochondrial genes; S; G2M phase score, identify most variable genes and run PCA on them
......@@ -24,15 +27,15 @@ Determining cellular heterogeneity in the human prostate with single-cell RNA se
* sc_PC.Score.NE (PCA analysis of Neurocrine Epithelia protein marker signature, projecction to PC1 used as "NE Score", highest scoring cells identified as Neuroendocrine Cells)
* sc_QuSAGE.LGEA.Epi (Epithelial clusters correlated to top 20 DEGs from scRNA-seq of human lung Basal Cells, Alveolar Type 2 Cells, Club/Goblet Cells to determine similarity to lung epithelial cell types)
* sc_QuSAGE.LGEA.St (Stromal clusters correlated to human orthologs of mouse lung stromal DEGs from scRNA*seq to determine similarity to:)
* Proliferative Fibrobblasts
* Myofibroblast/Smooth Muscle-Like Cells
* Matrix Fibroblasts
* Endothelial Cells
* Myeloid/Immune Cells
* Proliferative Fibrobblasts
* Myofibroblast/Smooth Muscle-Like Cells
* Matrix Fibroblasts
* Endothelial Cells
* Myeloid/Immune Cells
* sc_DEG (generate DEG lists between important cell types and predict surface and nuclear markers from them)
* sc_Table (produce tables of population differences between samples)
* Genesets
* **Genesets**:
* "regev\_lab\_cell\_cycle\_genes.txt" G2M and S phase genes from [*Genome Res. 2015 Dec; 25(12): 1860–1872*](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4665007/)
* "DEG\_C2.CGP.M10970.txt" MSigDB C2 Chemical and Genetic Perturbations M10970 [**CHUANG\_OXIDATIVE\_STRESS\_RESPONSE\_UP**](http://software.broadinstitute.org/gsea/msigdb/cards/CHUANG_OXIDATIVE_STRESS_RESPONSE_UP.html)
* "DEG\_Epi_2FC.txt" DWS generated DEGs of Epithelia from FACS population (bulk) RNA-sequencing
......@@ -48,10 +51,10 @@ Determining cellular heterogeneity in the human prostate with single-cell RNA se
* "Normal AT2 cells-signature-genes.csv" scRNA-Sequecing Lung Map generated top 20 DEGs for human lung Alveolar Type 2 Cells
* "Club\_Goblet cells-signature-genes.csv" scRNA-Sequencing Lung Map generated top 20 DEGs for human lung Club/Goblet Cells
* "journal.pcbi.1004575.s026.XLSX" scRNA-Sequencing Lung Map generated DEGs for E16.5 mouse lung stromal subtypes (genes converted to human othologs with Ensembl):
* Proliferative Fibroblasts
* Myofibroblast/Smooth Muscle-like Cells
* Pericytes, Matrix Fibroblasts
* Endothelial Cells
* Myeloid/Immune Cells
* Proliferative Fibroblasts
* Myofibroblast/Smooth Muscle-like Cells
* Pericytes, Matrix Fibroblasts
* Endothelial Cells
* Myeloid/Immune Cells
* "DWS.scStress.txt" DWS generated DEGs of Stressed Cells from scRNA-Sequencing
* "DWS.scNE.txt" DWS generated DEGs of Neuroendocrine Cells from scRNA-Sequencing
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