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BICF
Astrocyte
cellranger_mkfastq
Commits
b1e7748e
Commit
b1e7748e
authored
Jul 10, 2019
by
Gervaise Henry
🤠
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Update README.md
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@@ -25,27 +25,27 @@ To Run:
*
Available parameters:
*
**--name**
*
run name, puts outputs in a directory with this name
*
eg:
**--name 'test'**
*
run name, puts outputs in a directory with this name
*
eg:
**--name 'test'**
*
**--bcl**
*
Base call files (tarballed [
*.tar] +/- gunzipping [*
.tar.gz] from a sequencing of 10x single-cell expereiment, supports pigr parallelization).
*
There can be multiple basecall files, but they all will be demultiplexed by the same design file.
*
eg:
**--bcl '/project/shared/bicf_workflow_ref/workflow_testdata/cellranger/cellranger_mkfastq/simple1/cellranger-tiny-bcl-1_2_0.tar.gz'**
*
Base call files (tarballed [
*.tar] +/- gunzipping [*
.tar.gz] from a sequencing of 10x single-cell expereiment, supports pigr parallelization).
*
There can be multiple basecall files, but they all will be demultiplexed by the same design file.
*
eg:
**--bcl '/project/shared/bicf_workflow_ref/workflow_testdata/cellranger/cellranger_mkfastq/simple1/cellranger-tiny-bcl-1_2_0.tar.gz'**
*
**--designFile**
*
path to design file (csv format) location
*
column 1 = "Lane" (number of lanes to demultiplex,
*/*
*
for all lanes)
*
column 2 = "Sample" (sample name)
*
column 3 = "Index" (10x sample index barcode, eg SI-GA-A1)
*
can have repeated "Sample" if there are multiple fastq R1/R2 pairs for the samples
*
can be downloaded
[
HERE
](
https://git.biohpc.swmed.edu/BICF/Astrocyte/cellranger_mkfastq/blob/master/docs/design.csv
)
*
eg:
**--designFile '/project/shared/bicf_workflow_ref/workflow_testdata/cellranger/cellranger_mkfastq/simple/cellranger-tiny-bcl-simple-1_2_0.csv'**
*
**--outDir**
*
optional output directory for run
*
eg:
**--outDir 'test'**
*
FULL EXAMPLE:
**nextflow run workflow/main.nf --name 'test' --bcl '/project/shared/bicf_workflow_ref/workflow_testdata/cellranger/cellranger_mkfastq/simple1/cellranger-tiny-bcl-1_2_0.tar.gz' --designFile '/project/shared/bicf_workflow_ref/workflow_testdata/cellranger/cellranger_mkfastq/simple1/cellranger-tiny-bcl-simple-1_2_0.csv' --outDir 'test'**
*
path to design file (csv format) location
*
column 1 = "Lane" (number of lanes to demultiplex,
*/*
*
for all lanes)
*
column 2 = "Sample" (sample name)
*
column 3 = "Index" (10x sample index barcode, eg SI-GA-A1)
*
can have repeated "Sample" if there are multiple fastq R1/R2 pairs for the samples
*
can be downloaded
[
HERE
](
https://git.biohpc.swmed.edu/BICF/Astrocyte/cellranger_mkfastq/blob/master/docs/design.csv
)
*
eg:
**--designFile '/project/shared/bicf_workflow_ref/workflow_testdata/cellranger/cellranger_mkfastq/simple/cellranger-tiny-bcl-simple-1_2_0.csv'**
*
**--outDir**
*
optional output directory for run
*
eg:
**--outDir 'test'**
*
FULL EXAMPLE:
```
nextflow run workflow/main.nf --name 'test' --bcl '/project/shared/bicf_workflow_ref/workflow_testdata/cellranger/cellranger_mkfastq/simple1/cellranger-tiny-bcl-1_2_0.tar.gz' --designFile '/project/shared/bicf_workflow_ref/workflow_testdata/cellranger/cellranger_mkfastq/simple1/cellranger-tiny-bcl-simple-1_2_0.csv' --outDir 'test'
```
*
Design example:
| Lane | Sample | Index |
...
...
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