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cellranger_mkfastq
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BICF
Astrocyte
cellranger_mkfastq
Commits
7b474afc
There was a problem fetching the pipeline stages.
Commit
7b474afc
authored
6 years ago
by
Gervaise Henry
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Add fastqc (needs input file optimization)
parent
6e722372
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2 merge requests
!8
Develop
,
!6
Resolve "Add MultiQC"
Pipeline
#3462
passed with stages
in 1 minute and 21 seconds
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2
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workflow/main.nf
+28
-4
28 additions, 4 deletions
workflow/main.nf
workflow/scripts/fastqc.sh
+4
-0
4 additions, 0 deletions
workflow/scripts/fastqc.sh
with
32 additions
and
4 deletions
workflow/main.nf
+
28
−
4
View file @
7b474afc
...
...
@@ -81,7 +81,7 @@ process mkfastq {
output:
file("**/outs/fastq_path/**/*") into fastqPaths
file("**/outs/fastq_path/Stats/Stats.json") into qc
file("**/outs/fastq_path/Stats/Stats.json") into
b
qc
Paths
script:
...
...
@@ -94,16 +94,40 @@ process mkfastq {
"""
}
process fastqc {
publishDir "$outDir/${task.process}", mode: 'copy'
input:
file("outs/fastq_path/*/*") from fastqPaths
output:
file("*fastqc.*") into fqcPaths
script:
"""
hostname
ulimit -a
module load fastqc/0.11.5
module load parallel
sh $baseDir/scripts/fastqc.sh
"""
}
process multiqc {
publishDir "$outDir/${task.process}", mode: 'copy'
input:
file qc
file bqcPaths
file fqcPaths
output:
file("*") into qcPaths
file("*") into
m
qcPaths
script:
...
...
@@ -111,6 +135,6 @@ process multiqc {
hostname
ulimit -a
module load multiqc/1.7
multiqc
Stats.json
multiqc
.
"""
}
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workflow/scripts/fastqc.sh
0 → 100644
+
4
−
0
View file @
7b474afc
#!/bin/bash
find
.
-name
'*.fastq.gz'
|
awk
'{printf("fastqc \"%s\"\n", $0)}'
| parallel
-j
25
--verbose
find
.
-name
'*fastqc.*'
| xargs
-I
'{}'
mv
'{}'
./
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