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Commit cb957223 authored by Gervaise Henry's avatar Gervaise Henry :cowboy:
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Merge branch '17-documentation' into 'develop'

Resolve "Update Documentation"

Closes #17

See merge request !31
parents ea2289c2 6db012da
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3 merge requests!35Develop,!32Develop,!31Resolve "Update Documentation"
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|*master*|*develop*|
|:-:|:-:|
|[![Build Status](https://git.biohpc.swmed.edu/BICF/Astrocyte/cellranger_count/badges/master/build.svg)](https://git.biohpc.swmed.edu/BICF/Astrocyte/cellranger_count/commits/master)|[![Build Status](https://git.biohpc.swmed.edu/BICF/Astrocyte/cellranger_count/badges/develop/build.svg)](https://git.biohpc.swmed.edu/BICF/Astrocyte/cellranger_count/commits/develop)|
10x Genomics scRNA-Seq (cellranger) count Pipeline 10x Genomics scRNA-Seq (cellranger) count Pipeline
======================================== ==================================================
Introduction Introduction
------------ ------------
...@@ -92,4 +96,4 @@ To Run: ...@@ -92,4 +96,4 @@ To Run:
|---------|------------------------------------|------------------------------------| |---------|------------------------------------|------------------------------------|
| sample1 | pbmc_1k_v2_S1_L001_R1_001.fastq.gz | pbmc_1k_v2_S1_L001_R2_001.fastq.gz | | sample1 | pbmc_1k_v2_S1_L001_R1_001.fastq.gz | pbmc_1k_v2_S1_L001_R2_001.fastq.gz |
| sample2 | pbmc_1k_v2_S2_L001_R1_001.fastq.gz | pbmc_1k_v2_S2_L001_R2_001.fastq.gz | | sample2 | pbmc_1k_v2_S2_L001_R1_001.fastq.gz | pbmc_1k_v2_S2_L001_R2_001.fastq.gz |
| sample2 | pbmc_1k_v2_S2_L002_R1_001.fastq.gz | pbmc_1k_v2_S2_L002_R2_001.fastq.gz | | sample2 | pbmc_1k_v2_S2_L002_R1_001.fastq.gz | pbmc_1k_v2_S2_L002_R2_001.fastq.gz |
\ No newline at end of file
10x Genomics scRNA-Seq (cellranger) count Pipeline 10x Genomics scRNA-Seq (cellranger) count Pipeline
======================================== ==================================================
Introduction Introduction
------------ ------------
...@@ -24,7 +24,7 @@ To Run: ...@@ -24,7 +24,7 @@ To Run:
* column 3 = "fastq_R2" * column 3 = "fastq_R2"
* can have repeated "Sample" if there are multiole fastq R1/R2 pairs for the samples * can have repeated "Sample" if there are multiole fastq R1/R2 pairs for the samples
* eg: can be downloaded [HERE](https://git.biohpc.swmed.edu/BICF/Astrocyte/cellranger_count/blob/8db3e25c13cb1463c2a50e510159c72380ae5826/docs/design.csv) * eg: can be downloaded [HERE](https://git.biohpc.swmed.edu/BICF/Astrocyte/cellranger_count/blob/8db3e25c13cb1463c2a50e510159c72380ae5826/docs/design.csv)
* **genome** * **genome**
* Reference species and genome used for alignment and subsequent analysis. * Reference species and genome used for alignment and subsequent analysis.
* name of available 10x Gemomics premade reference genomes: * name of available 10x Gemomics premade reference genomes:
* *'GRCh38-3.0.0'* = Human GRCh38 release 93 * *'GRCh38-3.0.0'* = Human GRCh38 release 93
...@@ -36,30 +36,39 @@ To Run: ...@@ -36,30 +36,39 @@ To Run:
* *'hg19_and_mm10-3.0.0'* = Human GRCh37 (hg19) + Mouse GRCm38 (mm19) release 93 * *'hg19_and_mm10-3.0.0'* = Human GRCh37 (hg19) + Mouse GRCm38 (mm19) release 93
* *'hg19_and_mm10-1.2.0'* = Human GRCh37 (hg19) + Mouse GRCm38 (mm19) release 84 * *'hg19_and_mm10-1.2.0'* = Human GRCh37 (hg19) + Mouse GRCm38 (mm19) release 84
* *'ercc92-1.2.0'* = ERCC.92 Spike-In * *'ercc92-1.2.0'* = ERCC.92 Spike-In
* **expect cells** * **expect cells**
* Expected number of recovered cells. * Expected number of recovered cells.
* guides cellranger in it's cutoff for background/low quality cells * guides cellranger in it's cutoff for background/low quality cells
* as a guide it doesn't have to be exact * as a guide it doesn't have to be exact
* 0-10000 * 0-10000
* if --expextedCells is used then --forceCells is not necessary * if --expextedCells is used then --forceCells is not necessary
* only used if force cells is not entered or set to 0 * only used if force cells is not entered or set to 0
* **force cells** * **force cells**
* Force pipeline to use this number of cells, bypassing the cell detection algorithm. Use this if the number of cells estimated by Cell Ranger is not consistent with the barcode rank plot. A value of 0 ignores this option. Any value other than 0 overrides expect-cells. * Force pipeline to use this number of cells, bypassing the cell detection algorithm. Use this if the number of cells estimated by Cell Ranger is not consistent with the barcode rank plot. A value of 0 ignores this option. Any value other than 0 overrides expect-cells.
* 0-10000 * 0-10000
* if force cells is used then expected cells is not necessary and is ignored * if force cells is used then expected cells is not necessary and is ignored
* **chemistry version** * **chemistry version**
* 10x single cell gene expression chemistry version (only used in cellranger version 3.x). * 10x single cell gene expression chemistry version (only used in cellranger version 3.x).
* setting to auto will attempt to autodetect from the detected cycle strategy in the fastq's * setting to auto will attempt to autodetect from the detected cycle strategy in the fastq's
* chemistry version is only used if cellranger version is > 2.x * chemistry version is only used if cellranger version is > 2.x
* cellranger version 2.1.1 can only read chemistry version less than or equal to two (2) * cellranger version 2.1.1 can only read chemistry version less than or equal to two (2)
* **cellranger version** * **cellranger version**
* 10x cellranger version. * 10x cellranger version.
* cellranger version 2.1.1 can only read chemistry version less than or equal to two (2) * cellranger version 2.1.1 can only read chemistry version less than or equal to two (2)
* Design example: * Design example:
| Sample | fastq_R1 | fastq_R2 | | Sample | fastq_R1 | fastq_R2 |
|---------|------------------------------------|------------------------------------| |---------|------------------------------------|------------------------------------|
| sample1 | pbmc_1k_v2_S1_L001_R1_001.fastq.gz | pbmc_1k_v2_S1_L001_R2_001.fastq.gz | | sample1 | pbmc_1k_v2_S1_L001_R1_001.fastq.gz | pbmc_1k_v2_S1_L001_R2_001.fastq.gz |
| sample2 | pbmc_1k_v2_S2_L001_R1_001.fastq.gz | pbmc_1k_v2_S2_L001_R2_001.fastq.gz | | sample2 | pbmc_1k_v2_S2_L001_R1_001.fastq.gz | pbmc_1k_v2_S2_L001_R2_001.fastq.gz |
| sample2 | pbmc_1k_v2_S2_L002_R1_001.fastq.gz | pbmc_1k_v2_S2_L002_R2_001.fastq.gz | | sample2 | pbmc_1k_v2_S2_L002_R1_001.fastq.gz | pbmc_1k_v2_S2_L002_R2_001.fastq.gz |
Credits
-------
This worklow is was developed jointly with the [Bioinformatic Core Facility (BICF), Department of Bioinformatics](http://www.utsouthwestern.edu/labs/bioinformatics/)
Please cite in publications: Pipeline was developed by BICF from funding provided by **Cancer Prevention and Research Institute of Texas (RP150596)**.
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