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Astrocyte
cellranger_count
Commits
a7f754a7
Commit
a7f754a7
authored
5 years ago
by
Gervaise Henry
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Merge branch '26-ModuleLoad' into 'develop'
Module Load moved to top of nextflow processes Closes
#26
See merge request
!41
parents
baadbf42
185d1ef0
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2 merge requests
!53
Develop
,
!41
Module Load moved to top of nextflow processes
Pipeline
#3949
passed with stages
in 27 minutes and 22 seconds
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workflow/main.nf
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a7f754a7
...
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@@ -58,6 +58,7 @@ outDir = params.outDir
process checkDesignFile {
publishDir "$outDir/misc/${task.process}/$name", mode: 'copy'
module 'python/3.6.1-2-anaconda'
input:
...
...
@@ -73,7 +74,6 @@ process checkDesignFile {
"""
hostname
ulimit -a
module load python/3.6.1-2-anaconda
python3 $baseDir/scripts/check_design.py -d $designLocation -f $fastqList
"""
}
...
...
@@ -108,8 +108,8 @@ chemistryParam302 = chemistryParam
process count211 {
queue '128GB,256GB,256GBv1,384GB'
tag "$sample"
publishDir "$outDir/${task.process}", mode: 'copy'
module 'cellranger/2.1.1'
input:
...
...
@@ -130,14 +130,12 @@ process count211 {
"""
hostname
ulimit -a
module load cellranger/2.1.1
cellranger count --id="$sample" --transcriptome="./$ref" --fastqs=. --sample="$sample" --expect-cells=$expectCells211
"""
} else {
"""
hostname
ulimit -a
module load cellranger/2.1.1
cellranger count --id="$sample" --transcriptome="./$ref" --fastqs=. --sample="$sample" --force-cells=$forceCells211
"""
}
...
...
@@ -146,8 +144,8 @@ process count211 {
process count301 {
queue '128GB,256GB,256GBv1,384GB'
tag "$sample"
publishDir "$outDir/${task.process}", mode: 'copy'
module 'cellranger/3.0.1'
input:
...
...
@@ -169,14 +167,12 @@ process count301 {
"""
hostname
ulimit -a
module load cellranger/3.0.1
cellranger count --id="$sample" --transcriptome="./$ref" --fastqs=. --sample="$sample" --expect-cells=$expectCells301 --chemistry="$chemistryParam301"
"""
} else {
"""
hostname
ulimit -a
module load cellranger/3.0.1
cellranger count --id="$sample" --transcriptome="./$ref" --fastqs=. --sample="$sample" --force-cells=$forceCells301 --chemistry="$chemistryParam301"
"""
}
...
...
@@ -185,8 +181,8 @@ process count301 {
process count302 {
queue '128GB,256GB,256GBv1,384GB'
tag "$sample"
publishDir "$outDir/${task.process}", mode: 'copy'
module 'cellranger/3.0.2'
input:
...
...
@@ -208,14 +204,12 @@ process count302 {
"""
hostname
ulimit -a
module load cellranger/3.0.2
cellranger count --id="$sample" --transcriptome="./$ref" --fastqs=. --sample="$sample" --expect-cells=$expectCells302 --chemistry="$chemistryParam302"
"""
} else {
"""
hostname
ulimit -a
module load cellranger/3.0.2
cellranger count --id="$sample" --transcriptome="./$ref" --fastqs=. --sample="$sample" --force-cells=$forceCells302 --chemistry="$chemistryParam302"
"""
}
...
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