From d6c6fbbf4d1711db5948acc8c12f3a288dac767a Mon Sep 17 00:00:00 2001 From: Brandi Cantarel <brandi.cantarel@utsouthwestern.edu> Date: Mon, 6 May 2019 20:40:09 -0500 Subject: [PATCH] update markdups --- alignment/markdups.sh | 3 --- 1 file changed, 3 deletions(-) diff --git a/alignment/markdups.sh b/alignment/markdups.sh index 9a5eb6b..5687a5d 100644 --- a/alignment/markdups.sh +++ b/alignment/markdups.sh @@ -42,9 +42,6 @@ then sambamba markdup -t $SLURM_CPUS_ON_NODE ${sbam} ${pair_id}.dedup.bam elif [ $algo == 'samtools' ] then - samtools sort -n -@ $SLURM_CPUS_ON_NODE -o nsort.bam ${sbam} - samtools fixmate -c --output-fmt BAM -m -@ $SLURM_CPUS_ON_NODE nsort.bam fix.bam - samtools sort -n -@ $SLURM_CPUS_ON_NODE -o sort.bam fix.bam samtools markdup -s --output-fmt BAM -@ $SLURM_CPUS_ON_NODE sort.bam ${pair_id}.dedup.bam elif [ $algo == 'picard' ] then -- GitLab