diff --git a/alignment/bamqc.sh b/alignment/bamqc.sh index 2e9f460aafdb4ec47224ed0164140dfc51f36fcb..04de66d4e7a77739c29e9b81b19c62e50d60f87a 100644 --- a/alignment/bamqc.sh +++ b/alignment/bamqc.sh @@ -47,6 +47,7 @@ if [[ $nuctype == 'dna' ]]; then samtools view -b --threads $SLURM_CPUS_ON_NODE -L ${bed} -o ${pair_id}.ontarget.bam ${sbam} samtools index ${pair_id}.ontarget.bam samtools flagstat ${pair_id}.ontarget.bam > ${pair_id}.ontarget.flagstat.txt + samtools view -b -F 1024 ${pair_id}.ontarget.bam | bedtools coverage -sorted -g ${index_path}/genomefile.txt -a ${bed} -b stdin -hist | grep ^all > ${pair_id}.dedupcov.txt java -Xmx64g -jar $PICARD/picard.jar CollectInsertSizeMetrics INPUT=${sbam} HISTOGRAM_FILE=${pair_id}.hist.ps REFERENCE_SEQUENCE=${index_path}/genome.fa OUTPUT=${pair_id}.hist.txt java -Xmx64g -jar $PICARD/picard.jar CollectAlignmentSummaryMetrics R=${index_path}/genome.fa I=${sbam} OUTPUT=${pair_id}.alignmentsummarymetrics.txt java -Xmx64g -jar $PICARD/picard.jar EstimateLibraryComplexity I=${sbam} OUTPUT=${pair_id}.libcomplex.txt