diff --git a/alignment/bamqc.sh b/alignment/bamqc.sh index 86d1643549712eba4a4fbbfac8b12a42e981087c..82346c73e05ff9d593adff76a865070937136e5a 100644 --- a/alignment/bamqc.sh +++ b/alignment/bamqc.sh @@ -44,15 +44,15 @@ fi if [[ $nuctype == 'dna' ]]; then module load bedtools/2.26.0 picard/2.10.3 - samtools view -b --threads $SLURM_CPUS_ON_NODE -L ${bed} -o ${pair_id}.ontarget.bam ${sbam} - samtools index ${pair_id}.ontarget.bam + samtools view -@ $SLURM_CPUS_ON_NODE -b -L ${bed} -o ${pair_id}.ontarget.bam ${sbam} + samtools index -@ $SLURM_CPUS_ON_NODE ${pair_id}.ontarget.bam #samtools flagstat ${pair_id}.ontarget.bam > ${pair_id}.ontarget.flagstat.txt java -Xmx64g -jar $PICARD/picard.jar CollectInsertSizeMetrics INPUT=${sbam} HISTOGRAM_FILE=${pair_id}.hist.ps REFERENCE_SEQUENCE=${index_path}/genome.fa OUTPUT=${pair_id}.hist.txt java -Xmx64g -jar $PICARD/picard.jar CollectAlignmentSummaryMetrics R=${index_path}/genome.fa I=${pair_id}.ontarget.bam OUTPUT=${pair_id}.alignmentsummarymetrics.txt java -Xmx64g -jar $PICARD/picard.jar EstimateLibraryComplexity I=${sbam} OUTPUT=${pair_id}.libcomplex.txt - samtools view -b -q 1 ${sbam} | bedtools coverage -sorted -hist -g ${index_path}/genomefile.txt -b stdin -a ${bed} > ${pair_id}.mapqualcov.txt + samtools view -@ $SLURM_CPUS_ON_NODE -b -q 1 ${sbam} | bedtools coverage -sorted -hist -g ${index_path}/genomefile.txt -b stdin -a ${bed} > ${pair_id}.mapqualcov.txt bedtools coverage -sorted -g ${index_path}/genomefile.txt -a ${bed} -b ${sbam} -hist > ${pair_id}.covhist.txt grep ^all ${pair_id}.covhist.txt > ${pair_id}.genomecov.txt - samtools view ${sbam} | awk '{sum+=$5} END { print "Mean MAPQ =",sum/NR}' > ${pair_id}.meanmap.txt + samtools view -@ $SLURM_CPUS_ON_NODE ${sbam} | awk '{sum+=$5} END { print "Mean MAPQ =",sum/NR}' > ${pair_id}.meanmap.txt perl $baseDir/calculate_depthcov.pl ${pair_id}.covhist.txt fi diff --git a/alignment/starfusion.sh b/alignment/starfusion.sh index 070e8d3d51ed8432fa98fc089028447d250fcb57..86fc7ea832dd171907930ca21026ac04c21205bb 100644 --- a/alignment/starfusion.sh +++ b/alignment/starfusion.sh @@ -33,7 +33,7 @@ fi index_path=${refgeno}/CTAT_lib/ baseDir="`dirname \"$0\"`" source /etc/profile.d/modules.sh -module add python/2.7.x-anaconda star/2.5.2b +module add python/2.7.x-anaconda star/2.5.2b bedtools/2.26.0 STAR-Fusion --genome_lib_dir ${index_path} --left_fq ${fq1} --right_fq ${fq2} --output_dir star_fusion &> star_fusion.err mv star_fusion/star-fusion.fusion_candidates.final.abridged ${pair_id}.starfusion.txt