diff --git a/alignment/dnaseqalign.sh b/alignment/dnaseqalign.sh index d08cf71ae436be4b5e5455977910b5f2de5d0f41..aecb7e35b6e6f5bebbcb51ece8fd207790e2503f 100644 --- a/alignment/dnaseqalign.sh +++ b/alignment/dnaseqalign.sh @@ -59,7 +59,7 @@ fi if [[ $umi == 'umi' ]] && [[ $index_path == '/project/shared/bicf_workflow_ref/GRCh38' ]] then k8 /cm/shared/apps/bwakit/0.7.15/bwa-postalt.js -p tmphla ${index_path}/genome.fa.alt out.sam | python ${baseDir}/add_umi_sam.py -s - -o output.unsort.bam -elif [[ $index_path == '/project/shared/bicf_workflow_ref/GRCh38' ]] +elif [[ $index_path == '/project/shared/bicf_workflow_ref/human/GRCh38' ]] then k8 /cm/shared/apps/bwakit/0.7.15/bwa-postalt.js -p tmphla ${index_path}/genome.fa.alt out.sam| samtools view -1 - > output.unsort.bam elif [[ $umi == 'umi' ]] diff --git a/variants/annotvcf.sh b/variants/annotvcf.sh index 7bc785b9e608a0595fa454e065f809a4dca5a0b3..1f4d9720de5b41baf5b9b4dd3adaa0e807015c74 100755 --- a/variants/annotvcf.sh +++ b/variants/annotvcf.sh @@ -25,12 +25,12 @@ shift $(($OPTIND -1)) source /etc/profile.d/modules.sh module load python/2.7.x-anaconda bedtools/2.26.0 samtools/1.6 snpeff/4.3q -if [[ $index_path == '/project/shared/bicf_workflow_ref/GRCh38/hisat_index' ]] +if [[ $index_path == '/project/shared/bicf_workflow_ref/human/GRCh38/hisat_index' ]] then - index_path='/project/shared/bicf_workflow_ref/GRCh38' + index_path='/project/shared/bicf_workflow_ref/human/GRCh38' fi -if [[ $index_path == '/project/shared/bicf_workflow_ref/GRCh38' ]] +if [[ $index_path == '/project/shared/bicf_workflow_ref/human/GRCh38' ]] then tabix ${unionvcf} bcftools annotate -Oz -a ${index_path}/gnomad.txt.gz -h ${index_path}/gnomad.header -c CHROM,POS,REF,ALT,GNOMAD_HOM,GNOMAD_AF,AF_POPMAX -o ${pair_id}.gnomad.vcf.gz ${unionvcf} @@ -39,10 +39,10 @@ then java -Xmx10g -jar $SNPEFF_HOME/snpEff.jar -no-downstream -no-upstream -no-intergenic -lof -c $SNPEFF_HOME/snpEff.config GRCh38.86 ${pair_id}.repeat.vcf.gz | java -jar $SNPEFF_HOME/SnpSift.jar annotate -id ${index_path}/dbSnp.vcf.gz - | java -jar $SNPEFF_HOME/SnpSift.jar annotate -info CLNSIG,CLNDSDB,CLNDSDBID,CLNDBN,CLNREVSTAT,CLNACC ${index_path}/clinvar.vcf.gz - | java -jar $SNPEFF_HOME/SnpSift.jar annotate -info CNT ${index_path}/cosmic.vcf.gz - | java -Xmx10g -jar $SNPEFF_HOME/SnpSift.jar dbnsfp -v -db ${index_path}/dbNSFP.txt.gz - | bgzip > ${pair_id}.annot.vcf.gz tabix ${pair_id}.annot.vcf.gz else - if [[ $index_path == '/project/shared/bicf_workflow_ref/GRCm38' ]] + if [[ $index_path == '/project/shared/bicf_workflow_ref/mouse/GRCm38' ]] then snpeffvers='GRCh38.86' - elif [[ $index_path == '/project/shared/bicf_workflow_ref/GRCh37' ]] + elif [[ $index_path == '/project/shared/bicf_workflow_ref/human/GRCh37' ]] then snpeffvers='GRCh37.75' fi