gc() library(optparse) option_list=list( make_option("--count",action="store",type='character',help="Count File") ) opt=parse_args(OptionParser(option_list=option_list)) rm(option_list) if (!("count" %in% names(opt))){ print("No count file passed, exiting.") exit() } else if (!file.exists(opt$count)) { print("No count file passed, exiting.") exit() } repRID <- basename(gsub(".featureCounts","",opt$count)) count <- read.delim(opt$count, comment.char="#") # if featureCounts file changes structure, be sure to update count and Length columns below colnames(count)[7] <- "count" rpk <- count$count/count$Length/1000 scale <- sum(rpk)/1000000 tpm <- rpk/scale output <- cbind(count,tpm) colnames(output)[7] <- "count" write.table(output,file=paste0(repRID,".countTable.csv"),sep=",",row.names=FALSE,quote=FALSE)