diff --git a/CHANGELOG.md b/CHANGELOG.md index 02c9891f6f7810cc78068aa080dc749e9f781ae4..57b95447b438ca5d213c8ddc32d0917975aa618a 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -9,7 +9,7 @@ * Make pull references from BioHPC default (including in biohpc.config) * Start using new gudmaprbk dockerhub (images autobuilt) * Moved consistency checks to be fully python -* Added back parallel form of trim_galore and now use fastqc after trim step +* Changed order of steps so that fastqc is done after the trim step *Known Bugs* * Datahub reference pull uses dev.gudmap.org as source until referencencs are placed on production diff --git a/workflow/rna-seq.nf b/workflow/rna-seq.nf index aa6e8a10cc4086991f2850e728473e9c58c262a3..f12ec9b4ecf22eb203c2a89057b9e424fe82dc32 100644 --- a/workflow/rna-seq.nf +++ b/workflow/rna-seq.nf @@ -341,7 +341,7 @@ process trimData { output: path ("*.fq.gz") into fastqsTrim - path ("*.fastq.gz") into fastqs_fastqc + path ("*.fastq.gz") includeInput:true into fastqs_fastqc path ("*_trimming_report.txt") into trimQC path ("readLength.csv") into inferMetadata_readLength @@ -881,7 +881,7 @@ process alignData { } // Replicate rawBam for multiple process inputs -rawBam.set { +rawBam.into { rawBam_dedupData }