From 7cf8c4d23d881383c1b15e25ea44f91f5c9fa78a Mon Sep 17 00:00:00 2001 From: Venkat Malladi <venkat.malladi@utsouthwestern.edu> Date: Sat, 31 Oct 2020 21:13:03 -0500 Subject: [PATCH] Update config for email. --- .gitlab-ci.yml | 12 ++++++------ README.md | 3 ++- workflow/nextflow.config | 3 ++- workflow/rna-seq.nf | 15 +++++++++++++-- 4 files changed, 23 insertions(+), 10 deletions(-) diff --git a/.gitlab-ci.yml b/.gitlab-ci.yml index 63a773a..20659ed 100644 --- a/.gitlab-ci.yml +++ b/.gitlab-ci.yml @@ -130,7 +130,7 @@ dedupData: - singularity run 'docker://bicf/gudmaprbkdedup:2.0.0' samtools sort -@ 20 -O BAM -o Q-Y5F6_1M.se.sorted.deduped.bam ./test_data/bam/small/Q-Y5F6_1M.se.deduped.bam - singularity run 'docker://bicf/gudmaprbkdedup:2.0.0' samtools index -@ 20 -b ./test_data/bam/small/Q-Y5F6_1M.se.sorted.deduped.bam Q-Y5F6_1M.se.sorted.deduped.bam.bai - > - for i in {"chr8","chr4","chrY"}; do + for i in {"chr8","chr4","chrY"}; do echo "samtools view -b Q-Y5F6_1M.se.sorted.deduped.bam ${i} > Q-Y5F6_1M.se.sorted.deduped.${i}.bam; samtools index -@ 20 -b Q-Y5F6_1M.se.sorted.deduped.${i}.bam Q-Y5F6_1M.se.sorted.deduped.${i}.bam.bai;"; done | singularity run 'docker://bicf/gudmaprbkdedup:2.0.0' parallel -j 20 -k - pytest -m dedupData @@ -145,7 +145,7 @@ countData: script: - ln -s /project/BICF/BICF_Core/shared/gudmap/references/GRCh38.p12.v31/geneID.tsv - ln -s /project/BICF/BICF_Core/shared/gudmap/references/GRCh38.p12.v31/Entrez.tsv - - singularity run 'docker://bicf/subread2:2.0.0' featureCounts -T 20 -a /project/BICF/BICF_Core/shared/gudmap/references/GRCh38.p12.v31/genome.gtf -G /project/BICF/BICF_Core/shared/gudmap/references/GRCh38.p12.v31/genome.fna -g 'gene_name' --extraAttributes 'gene_id' -o Q-Y5F6_1M.se.countData -s 1 -R SAM --primary --ignoreDup ./test_data/bam/small/Q-Y5F6_1M.se.sorted.deduped.bam + - singularity run 'docker://bicf/subread2:2.0.0' featureCounts -T 20 -a /project/BICF/BICF_Core/shared/gudmap/references/GRCh38.p12.v31/genome.gtf -G /project/BICF/BICF_Core/shared/gudmap/references/GRCh38.p12.v31/genome.fna -g 'gene_name' --extraAttributes 'gene_id' -o Q-Y5F6_1M.se.countData -s 1 -R SAM --primary --ignoreDup ./test_data/bam/small/Q-Y5F6_1M.se.sorted.deduped.bam - singularity run 'docker://bicf/subread2:2.0.0' Rscript ./workflow/scripts/calculateTPM.R --count ./test_data/counts/small/Q-Y5F6_1M.se.countData - singularity run 'docker://bicf/subread2:2.0.0' Rscript ./workflow/scripts/convertGeneSymbols.R --repRID Q-Y5F6_1M.se - assignedReads=$(grep -m 1 'Assigned' *.summary | grep -oe '\([0-9.]*\)') @@ -283,7 +283,7 @@ integration_se: script: - hostname - ulimit -a - - nextflow -q run ./workflow/rna-seq.nf --deriva ./test_data/auth/credential.json --bdbag ./test_data/auth/cookies.txt --repRID 16-1ZX4 -with-dag dag.png --ci true + - nextflow -q run ./workflow/rna-seq.nf --deriva ./test_data/auth/credential.json --bdbag ./test_data/auth/cookies.txt --repRID 16-1ZX4 -with-dag dag.png --ci true --email 'venkat.malladi@utsouthwestern.edu,Gervaise.Henry@UTSouthwestern.edu' - find . -type f -name "multiqc_data.json" -exec cp {} ./SE_multiqc_data.json \; artifacts: name: "$CI_JOB_NAME" @@ -366,7 +366,7 @@ override_fastq: max: 1 when: - always - + override_species: stage: integration only: [merge_requests] @@ -388,7 +388,7 @@ override_species: max: 1 when: - always - + consistency: stage: consistency @@ -413,4 +413,4 @@ consistency: - assignedPE.txt - assignedExpectSE.txt - assignedExpectPE.txt - expire_in: 7 days \ No newline at end of file + expire_in: 7 days diff --git a/README.md b/README.md index e3134bf..021a60b 100644 --- a/README.md +++ b/README.md @@ -43,6 +43,7 @@ To Run: * **biohpc_max** = process on high power BioHPC cluster nodes (=> 128GB nodes), for resource testing * **aws_ondemand** = AWS Batch on-demand instant requests * **aws_spot** = AWS Batch spot instance requests + * `-email`x email notification on failure * NOTES: * once deriva-auth is run and authenticated, the two files above are saved in ```~/.deriva/``` (see official documents from [deriva](https://github.com/informatics-isi-edu/deriva-client#installer-packages-for-windows-and-macosx) on the lifetime of the credentials) * reference version consists of Genome Reference Consortium version, patch release and GENCODE annotation release # (leaving the params blank will use the default version tied to the pipeline version) @@ -126,4 +127,4 @@ Please cite in publications: Pipeline was developed by BICF from funding provide Pipeline Directed Acyclic Graph ------------------------------- - \ No newline at end of file + diff --git a/workflow/nextflow.config b/workflow/nextflow.config index 218cf2a..f9fcef9 100644 --- a/workflow/nextflow.config +++ b/workflow/nextflow.config @@ -82,7 +82,7 @@ timeline { enabled = false file = 'timeline.html' } - + report { enabled = false file = 'report.html' @@ -94,6 +94,7 @@ tower { } manifest { + name = 'gudmap_rbk/rna-seq' homePage = 'https://git.biohpc.swmed.edu/gudmap_rbk/rna-seq' description = 'This pipeline was created to be a standard mRNA-sequencing analysis pipeline which integrates with the GUDMAP and RBK consortium data-hub.' mainScript = 'rna-seq.nf' diff --git a/workflow/rna-seq.nf b/workflow/rna-seq.nf index 3099e99..0ca7965 100644 --- a/workflow/rna-seq.nf +++ b/workflow/rna-seq.nf @@ -18,6 +18,8 @@ params.refMoVersion = "38.p6.vM22" params.refHuVersion = "38.p12.v31" params.refERCCVersion = "92" params.outDir = "${baseDir}/../output" +params.email = "" + // Define override input variable params.refSource = "biohpc" @@ -25,6 +27,7 @@ params.inputBagForce = "" params.fastqsForce = "" params.speciesForce = "" + // Parse input variables deriva = Channel .fromPath(params.deriva) @@ -46,6 +49,7 @@ logsDir = "${outDir}/Logs" inputBagForce = params.inputBagForce fastqsForce = params.fastqsForce speciesForce = params.speciesForce +email = params.email // Define fixed files derivaConfig = Channel.fromPath("${baseDir}/conf/replicate_export_config.json") @@ -1241,16 +1245,23 @@ process outputBag { """ } + workflow.onError = { + subject = "$$workflow.manifest.name FAILED: $params.repRID" + def msg = """\ Pipeline error summary --------------------------- RID : ${params.repRID} + Version : ${workflow.manifest.version} + Duration : ${workflow.duration} + Nf Version : ${workflow.nextflow.version} Message : ${workflow.errorMessage} exit status : ${workflow.exitStatus} """ .stripIndent() - - sendMail(to: 'venkat.malladi@utsouthwestern.edu', subject: 'GUDMAP RNA-seq error execution', body: msg) + if (email != '') { + sendMail(to: email, subject: subject , body: msg) + } } -- GitLab