diff --git a/test_data/createTestData.sh b/test_data/createTestData.sh index 8f5cebc6180a739189693c62451a3eb0f1970245..8f58771bf5a5d3b92f64dc9f35ee79b04f6608bf 100644 --- a/test_data/createTestData.sh +++ b/test_data/createTestData.sh @@ -17,7 +17,7 @@ cp Q-Y5F6_inputBag.zip ./NEW_test_data/bag/Q-Y5F6_inputBag_xxxxxxxx.zip mkdir -p ./NEW_test_data/fastq unzip ./NEW_test_data/bag/Q-Y5F6_inputBag_xxxxxxxx.zip -singularity run 'docker://gudmaprbk/deriva1.3:1.0.0' bash ../workflow/scripts/bdbagFetch.sh Q-Y5F6_inputBag Q-Y5F6 +singularity run 'docker://gudmaprbk/deriva1.3:1.0.0' bash ../workflow/scripts/bdbag_fetch.sh Q-Y5F6_inputBag Q-Y5F6 cp Q-Y5F6.R1.fastq.gz ./NEW_test_data/fastq/Q-Y5F6.R1.fastq.gz cp Q-Y5F6.R2.fastq.gz ./NEW_test_data/fastq/Q-Y5F6.R2.fastq.gz @@ -45,11 +45,11 @@ cp metaTest.csv ./NEW_test_data/meta/metaTest.csv mkdir -p ./NEW_test_data/bam mkdir -p ./NEW_test_data/bam/small -singularity run 'docker://gudmaprbk/hisat2.2.1:1.0.0' hisat2 -p 20 --add-chrname --un-gz Q-Y5F6_1M.se.unal.gz -S Q-Y5F6_1M.se.sam -x /project/BICF/BICF_Core/shared/gudmap/references/GRCm38.p6.vM22/hisat2/genome --rna-strandness F -U ./NEW_test_data/fastq/small/Q-Y5F6_1M.se_trimmed.fq.gz --summary-file Q-Y5F6_1M.se.alignSummary.txt --new-summary +singularity run 'docker://gudmaprbk/hisat2.2.1:1.0.0' hisat2 -p 20 --add-chrname --un-gz Q-Y5F6_1M.se.unal.gz -S Q-Y5F6_1M.se.sam -x /project/BICF/BICF_Core/shared/gudmap/references/new/GRCm38.p6.vM25/hisat2/genome --rna-strandness F -U ./NEW_test_data/fastq/small/Q-Y5F6_1M.se_trimmed.fq.gz --summary-file Q-Y5F6_1M.se.alignSummary.txt --new-summary singularity run 'docker://gudmaprbk/hisat2.2.1:1.0.0' samtools view -1 -@ 20 -F 4 -F 8 -F 256 -o Q-Y5F6_1M.se.bam Q-Y5F6_1M.se.sam singularity run 'docker://gudmaprbk/hisat2.2.1:1.0.0' samtools sort -@ 20 -O BAM -o Q-Y5F6_1M.se.sorted.bam Q-Y5F6_1M.se.bam singularity run 'docker://gudmaprbk/hisat2.2.1:1.0.0' samtools index -@ 20 -b Q-Y5F6_1M.se.sorted.bam Q-Y5F6_1M.se.sorted.bam.bai -singularity run 'docker://gudmaprbk/hisat2.2.1:1.0.0' hisat2 -p 20 --add-chrname --un-gz Q-Y5F6_1M.pe.unal.gz -S Q-Y5F6_1M.pe.sam -x /project/BICF/BICF_Core/shared/gudmap/references/GRCm38.p6.vM22/hisat2/genome --rna-strandness FR --no-mixed --no-discordant -1 ./NEW_test_data/fastq/small/Q-Y5F6_1M.pe_val_1.fq.gz -2 ./NEW_test_data/fastq/small/Q-Y5F6_1M.pe_val_2.fq.gz --summary-file Q-Y5F6_1M.pe.alignSummary.txt --new-summary +singularity run 'docker://gudmaprbk/hisat2.2.1:1.0.0' hisat2 -p 20 --add-chrname --un-gz Q-Y5F6_1M.pe.unal.gz -S Q-Y5F6_1M.pe.sam -x /project/BICF/BICF_Core/shared/gudmap/references/new/GRCm38.p6.vM25/hisat2/genome --rna-strandness FR --no-mixed --no-discordant -1 ./NEW_test_data/fastq/small/Q-Y5F6_1M.pe_val_1.fq.gz -2 ./NEW_test_data/fastq/small/Q-Y5F6_1M.pe_val_2.fq.gz --summary-file Q-Y5F6_1M.pe.alignSummary.txt --new-summary singularity run 'docker://gudmaprbk/hisat2.2.1:1.0.0' samtools view -1 -@ 20 -F 4 -F 8 -F 256 -o Q-Y5F6_1M.pe.bam Q-Y5F6_1M.pe.sam singularity run 'docker://gudmaprbk/hisat2.2.1:1.0.0' samtools sort -@ 20 -O BAM -o Q-Y5F6_1M.pe.sorted.bam Q-Y5F6_1M.pe.bam singularity run 'docker://gudmaprbk/hisat2.2.1:1.0.0' samtools index -@ 20 -b Q-Y5F6_1M.pe.sorted.bam Q-Y5F6_1M.pe.sorted.bam.bai @@ -82,9 +82,9 @@ cp Q-Y5F6_1M.se.sorted.deduped.chrY.bam.bai ./NEW_test_data/bam/small/Q-Y5F6_1M. mkdir -p ./NEW_test_data/counts mkdir -p ./NEW_test_data/counts/small -ln -s /project/BICF/BICF_Core/shared/gudmap/references/GRCm38.p6.vM22/geneID.tsv -ln -s /project/BICF/BICF_Core/shared/gudmap/references/GRCm38.p6.vM22/Entrez.tsv -singularity run 'docker://gudmaprbk/subread2.0.1:1.0.0' featureCounts -T 20 -a /project/BICF/BICF_Core/shared/gudmap/references/GRCm38.p6.vM22/genome.gtf -G /project/BICF/BICF_Core/shared/gudmap/references/GRCm38.p6.vM22/genome.fna -g 'gene_name' --extraAttributes 'gene_id' -o Q-Y5F6_1M.se_countData -s 1 -R SAM --primary --ignoreDup ./NEW_test_data/bam/small/Q-Y5F6_1M.se.sorted.deduped.bam +ln -s /project/BICF/BICF_Core/shared/gudmap/references/new/GRCm38.p6.vM25/metadata/geneID.tsv +ln -s /project/BICF/BICF_Core/shared/gudmap/references/new/GRCm38.p6.vM25/metadata/Entrez.tsv +singularity run 'docker://gudmaprbk/subread2.0.1:1.0.0' featureCounts -T 20 -a /project/BICF/BICF_Core/shared/gudmap/references/new/GRCm38.p6.vM25/annotation/genome.gtf -G /project/BICF/BICF_Core/shared/gudmap/references/new/GRCm38.p6.vM25/sequence/genome.fna -g 'gene_name' --extraAttributes 'gene_id' -o Q-Y5F6_1M.se_countData -s 1 -R SAM --primary --ignoreDup ./NEW_test_data/bam/small/Q-Y5F6_1M.se.sorted.deduped.bam singularity run 'docker://gudmaprbk/subread2.0.1:1.0.0' Rscript ../workflow/scripts/calculateTPM.R --count Q-Y5F6_1M.se_countData singularity run 'docker://gudmaprbk/subread2.0.1:1.0.0' Rscript ../workflow/scripts/convertGeneSymbols.R --repRID Q-Y5F6_1M.se cp Q-Y5F6_1M.se_countData ./NEW_test_data/counts/small/Q-Y5F6_1M.se_countData @@ -104,7 +104,7 @@ cp Q-Y5F6_1M.R1_fastqc.zip ./NEW_test_data/fastqc/small/Q-Y5F6_1M.R1_fastqc.zip echo -e "geneID\tchrom\ttx_start\ttx_end\tTIN" > Q-Y5F6_1M.se.sorted.deduped.tin.xls for i in {"chr8","chr4","chrY"}; do -echo "tin.py -i ./NEW_test_data/bam/small/Q-Y5F6_1M.se.sorted.deduped.${i}.bam -r /project/BICF/BICF_Core/shared/gudmap/references/GRCm38.p6.vM22/bed/genome.bed; cat Q-Y5F6_1M.se.sorted.deduped.${i}.tin.xls | tr -s \"\\w\" \"\\t\" | grep -P \"\\t${i}\\t\";"; done | singularity run 'docker://gudmaprbk/rseqc4.0.0:1.0.0' parallel -j 20 -k >> Q-Y5F6_1M.se.sorted.deduped.tin.xls +echo "tin.py -i ./NEW_test_data/bam/small/Q-Y5F6_1M.se.sorted.deduped.${i}.bam -r /project/BICF/BICF_Core/shared/gudmap/references/new/GRCm38.p6.vM25/annotation/genome.bed; cat Q-Y5F6_1M.se.sorted.deduped.${i}.tin.xls | tr -s \"\\w\" \"\\t\" | grep -P \"\\t${i}\\t\";"; done | singularity run 'docker://gudmaprbk/rseqc4.0.0:1.0.0' parallel -j 20 -k >> Q-Y5F6_1M.se.sorted.deduped.tin.xls cp Q-Y5F6_1M.se.sorted.deduped.tin.xls ./NEW_test_data/meta/Q-Y5F6_1M.se.sorted.deduped.tin.xls chgrp -R BICF_Core ./NEW_test_data