diff --git a/workflow/rna-seq.nf b/workflow/rna-seq.nf
index 1aa409b103883e7eed3bdb981e294c5e1ce02201..c850e5b25cb69327c55ecf49566f393c288bb23b 100755
--- a/workflow/rna-seq.nf
+++ b/workflow/rna-seq.nf
@@ -157,6 +157,7 @@ endsManual = Channel.create()
 stranded = Channel.create()
 spike = Channel.create()
 species = Channel.create()
+reference = file ("/project/BICF/BICF_Core/s181706/github/gudmap/rna-seq/References/GRCh38.p12/hisat2")
 metadata.splitCsv(sep: ',', header: false).separate(
   rep,
   endsMeta,
@@ -249,4 +250,4 @@ process alignReads {
     samtools view -1 --threads `nproc` -o ${repRID}.bam ${repRID}.sam 1>${repRID}.align.out 2> ${repRID}.align.err;
     samtools sort -@ `nproc` -O BAM ${repRID}.bam 1>${repRID}.align.out 2> ${repRID}.align.err;
     """
-}
\ No newline at end of file
+}