From 4bd19e37f556149c92eed1465072782d9b30a9ed Mon Sep 17 00:00:00 2001
From: "Gervaise H. Henry" <gervaise.henry@utsouthwestern.edu>
Date: Fri, 19 Feb 2021 17:22:22 -0600
Subject: [PATCH] Move non nextflow configs back to workflow and symlink files
---
nextflow.config | 16 +-
{conf => nextflowConf}/.gitkeep | 0
.../Execution_Run_For_Output_Bag.json | 0
.../Replicate_For_Input_Bag.json | 0
{conf => nextflowConf}/aws.config | 0
{conf => nextflowConf}/bdbag.json | 0
{conf => nextflowConf}/biohpc.config | 0
{conf => nextflowConf}/biohpc_local.config | 0
{conf => nextflowConf}/biohpc_max.config | 0
{conf => nextflowConf}/dnanexus.config | 0
{conf => nextflowConf}/local.config | 0
{conf => nextflowConf}/multiqc_config.yaml | 0
{conf => nextflowConf}/ondemand.config | 0
{conf => nextflowConf}/spot.config | 0
workflow/conf/.gitkeep | 0
.../conf/Execution_Run_For_Output_Bag.json | 64 +++++++
workflow/conf/Replicate_For_Input_Bag.json | 97 ++++++++++
workflow/conf/bdbag.json | 28 +++
workflow/conf/multiqc_config.yaml | 180 ++++++++++++++++++
workflow/nextflow.config | 1 +
workflow/nextflowConf | 1 +
workflow/rna-seq.nf | 1 +
22 files changed, 380 insertions(+), 8 deletions(-)
rename {conf => nextflowConf}/.gitkeep (100%)
rename {conf => nextflowConf}/Execution_Run_For_Output_Bag.json (100%)
rename {conf => nextflowConf}/Replicate_For_Input_Bag.json (100%)
rename {conf => nextflowConf}/aws.config (100%)
rename {conf => nextflowConf}/bdbag.json (100%)
rename {conf => nextflowConf}/biohpc.config (100%)
rename {conf => nextflowConf}/biohpc_local.config (100%)
rename {conf => nextflowConf}/biohpc_max.config (100%)
rename {conf => nextflowConf}/dnanexus.config (100%)
rename {conf => nextflowConf}/local.config (100%)
rename {conf => nextflowConf}/multiqc_config.yaml (100%)
rename {conf => nextflowConf}/ondemand.config (100%)
rename {conf => nextflowConf}/spot.config (100%)
create mode 100644 workflow/conf/.gitkeep
create mode 100755 workflow/conf/Execution_Run_For_Output_Bag.json
create mode 100644 workflow/conf/Replicate_For_Input_Bag.json
create mode 100644 workflow/conf/bdbag.json
create mode 100644 workflow/conf/multiqc_config.yaml
create mode 120000 workflow/nextflow.config
create mode 120000 workflow/nextflowConf
create mode 120000 workflow/rna-seq.nf
diff --git a/nextflow.config b/nextflow.config
index 927e605..288fc9d 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -1,23 +1,23 @@
profiles {
standard {
- includeConfig 'conf/biohpc.config'
+ includeConfig 'nextflowConf/biohpc.config'
}
biohpc {
- includeConfig 'conf/biohpc.config'
+ includeConfig 'nextflowConf/biohpc.config'
}
biohpc_max {
- includeConfig 'conf/biohpc_max.config'
+ includeConfig 'nextflowConf/biohpc_max.config'
}
aws_ondemand {
- includeConfig 'conf/aws.config'
- includeConfig 'conf/ondemand.config'
+ includeConfig 'nextflowConf/aws.config'
+ includeConfig 'nextflowConf/ondemand.config'
}
aws_spot {
- includeConfig 'conf/aws.config'
- includeConfig 'conf/spot.config'
+ includeConfig 'nextflowConf/aws.config'
+ includeConfig 'nextflowConf/spot.config'
}
dnanexus {
- includeConfig 'conf/dnanexus.config'
+ includeConfig 'nextflowConf/dnanexus.config'
}
}
diff --git a/conf/.gitkeep b/nextflowConf/.gitkeep
similarity index 100%
rename from conf/.gitkeep
rename to nextflowConf/.gitkeep
diff --git a/conf/Execution_Run_For_Output_Bag.json b/nextflowConf/Execution_Run_For_Output_Bag.json
similarity index 100%
rename from conf/Execution_Run_For_Output_Bag.json
rename to nextflowConf/Execution_Run_For_Output_Bag.json
diff --git a/conf/Replicate_For_Input_Bag.json b/nextflowConf/Replicate_For_Input_Bag.json
similarity index 100%
rename from conf/Replicate_For_Input_Bag.json
rename to nextflowConf/Replicate_For_Input_Bag.json
diff --git a/conf/aws.config b/nextflowConf/aws.config
similarity index 100%
rename from conf/aws.config
rename to nextflowConf/aws.config
diff --git a/conf/bdbag.json b/nextflowConf/bdbag.json
similarity index 100%
rename from conf/bdbag.json
rename to nextflowConf/bdbag.json
diff --git a/conf/biohpc.config b/nextflowConf/biohpc.config
similarity index 100%
rename from conf/biohpc.config
rename to nextflowConf/biohpc.config
diff --git a/conf/biohpc_local.config b/nextflowConf/biohpc_local.config
similarity index 100%
rename from conf/biohpc_local.config
rename to nextflowConf/biohpc_local.config
diff --git a/conf/biohpc_max.config b/nextflowConf/biohpc_max.config
similarity index 100%
rename from conf/biohpc_max.config
rename to nextflowConf/biohpc_max.config
diff --git a/conf/dnanexus.config b/nextflowConf/dnanexus.config
similarity index 100%
rename from conf/dnanexus.config
rename to nextflowConf/dnanexus.config
diff --git a/conf/local.config b/nextflowConf/local.config
similarity index 100%
rename from conf/local.config
rename to nextflowConf/local.config
diff --git a/conf/multiqc_config.yaml b/nextflowConf/multiqc_config.yaml
similarity index 100%
rename from conf/multiqc_config.yaml
rename to nextflowConf/multiqc_config.yaml
diff --git a/conf/ondemand.config b/nextflowConf/ondemand.config
similarity index 100%
rename from conf/ondemand.config
rename to nextflowConf/ondemand.config
diff --git a/conf/spot.config b/nextflowConf/spot.config
similarity index 100%
rename from conf/spot.config
rename to nextflowConf/spot.config
diff --git a/workflow/conf/.gitkeep b/workflow/conf/.gitkeep
new file mode 100644
index 0000000..e69de29
diff --git a/workflow/conf/Execution_Run_For_Output_Bag.json b/workflow/conf/Execution_Run_For_Output_Bag.json
new file mode 100755
index 0000000..5945b1e
--- /dev/null
+++ b/workflow/conf/Execution_Run_For_Output_Bag.json
@@ -0,0 +1,64 @@
+{
+ "bag": {
+ "bag_name": "Execution_Run_{rid}",
+ "bag_algorithms": [
+ "md5"
+ ],
+ "bag_archiver": "zip",
+ "bag_metadata": {}
+ },
+ "catalog": {
+ "catalog_id": "2",
+ "query_processors": [
+ {
+ "processor": "csv",
+ "processor_params": {
+ "output_path": "Execution_Run",
+ "query_path": "/attribute/M:=RNASeq:Execution_Run/RID=17-BPAG/RID,Replicate_RID:=Replicate,Workflow_RID:=Workflow,Reference_Genone_RID:=Reference_Genome,Input_Bag_RID:=Input_Bag,Notes,Execution_Status,Execution_Status_Detail,RCT,RMT?limit=none"
+ }
+ },
+ {
+ "processor": "csv",
+ "processor_params": {
+ "output_path": "Workflow",
+ "query_path": "/entity/M:=RNASeq:Execution_Run/RID=17-BPAG/RNASeq:Workflow?limit=none"
+ }
+ },
+ {
+ "processor": "csv",
+ "processor_params": {
+ "output_path": "Reference_Genome",
+ "query_path": "/entity/M:=RNASeq:Execution_Run/RID=17-BPAG/RNASeq:Reference_Genome?limit=none"
+ }
+ },
+ {
+ "processor": "csv",
+ "processor_params": {
+ "output_path": "Input_Bag",
+ "query_path": "/entity/M:=RNASeq:Execution_Run/RID=17-BPAG/RNASeq:Input_Bag?limit=none"
+ }
+ },
+ {
+ "processor": "csv",
+ "processor_params": {
+ "output_path": "mRNA_QC",
+ "query_path": "/attribute/M:=RNASeq:Execution_Run/RID=17-BPAG/(RID)=(RNASeq:mRNA_QC:Execution_Run)/RID,Execution_Run_RID:=Execution_Run,Replicate_RID:=Replicate,Paired_End,Strandedness,Median_Read_Length,Raw_Count,Final_Count,Notes,RCT,RMT?limit=none"
+ }
+ },
+ {
+ "processor": "fetch",
+ "processor_params": {
+ "output_path": "assets/Study/{Study_RID}/Experiment/{Experiment_RID}/Replicate/{Replicate_RID}/Execution_Run/{Execution_Run_RID}/Output_Files",
+ "query_path": "/attribute/M:=RNASeq:Execution_Run/RID=17-BPAG/R:=RNASeq:Replicate/$M/(RID)=(RNASeq:Processed_File:Execution_Run)/url:=File_URL,length:=File_Bytes,filename:=File_Name,md5:=File_MD5,Execution_Run_RID:=M:RID,Study_RID:=R:Study_RID,Experiment_RID:=R:Experiment_RID,Replicate_RID:=R:RID?limit=none"
+ }
+ },
+ {
+ "processor": "fetch",
+ "processor_params": {
+ "output_path": "assets/Study/{Study_RID}/Experiment/{Experiment_RID}/Replicate/{Replicate_RID}/Execution_Run/{Execution_Run_RID}/Input_Bag",
+ "query_path": "/attribute/M:=RNASeq:Execution_Run/RID=17-BPAG/R:=RNASeq:Replicate/$M/RNASeq:Input_Bag/url:=File_URL,length:=File_Bytes,filename:=File_Name,md5:=File_MD5,Execution_Run_RID:=M:RID,Study_RID:=R:Study_RID,Experiment_RID:=R:Experiment_RID,Replicate_RID:=R:RID?limit=none"
+ }
+ }
+ ]
+ }
+}
\ No newline at end of file
diff --git a/workflow/conf/Replicate_For_Input_Bag.json b/workflow/conf/Replicate_For_Input_Bag.json
new file mode 100644
index 0000000..508a024
--- /dev/null
+++ b/workflow/conf/Replicate_For_Input_Bag.json
@@ -0,0 +1,97 @@
+{
+ "bag": {
+ "bag_name": "{rid}_inputBag",
+ "bag_algorithms": [
+ "md5"
+ ],
+ "bag_archiver": "zip"
+ },
+ "catalog": {
+ "query_processors": [
+ {
+ "processor": "csv",
+ "processor_params": {
+ "output_path": "Study",
+ "query_path": "/attribute/M:=RNASeq:Replicate/RID={rid}/(Study_RID)=(RNASeq:Study:RID)/Study_RID:=RID,Internal_ID,Title,Summary,Overall_Design,GEO_Series_Accession_ID,GEO_Platform_Accession_ID,Funding,Pubmed_ID,Principal_Investigator,Consortium,Release_Date,RCT,RMT?limit=none"
+ }
+ },
+ {
+ "processor": "csv",
+ "processor_params": {
+ "output_path": "Experiment",
+ "query_path": "/attribute/M:=RNASeq:Replicate/RID={rid}/(Experiment_RID)=(RNASeq:Experiment:RID)/Experiment_RID:=RID,Study_RID,Internal_ID,Name,Description,Experiment_Method,Experiment_Type,Species,Specimen_Type,Molecule_Type,Pooled_Sample,Pool_Size,Markers,Cell_Count,Treatment_Protocol,Treatment_Protocol_Reference,Isolation_Protocol,Isolation_Protocol_Reference,Growth_Protocol,Growth_Protocol_Reference,Label_Protocol,Label_Protocol_Reference,Hybridization_Protocol,Hybridization_Protocol_Reference,Scan_Protocol,Scan_Protocol_Reference,Data_Processing,Value_Definition,Notes,Principal_Investigator,Consortium,Release_Date,RCT,RMT?limit=none"
+ }
+ },
+ {
+ "processor": "csv",
+ "processor_params": {
+ "output_path": "Experiment Antibodies",
+ "query_path": "/entity/M:=RNASeq:Replicate/RID={rid}/(Experiment_RID)=(RNASeq:Experiment_Antibodies:Experiment_RID)?limit=none"
+ }
+ },
+ {
+ "processor": "csv",
+ "processor_params": {
+ "output_path": "Experiment Custom Metadata",
+ "query_path": "/entity/M:=RNASeq:Replicate/RID={rid}/(Experiment_RID)=(RNASeq:Experiment_Custom_Metadata:Experiment_RID)?limit=none"
+ }
+ },
+ {
+ "processor": "csv",
+ "processor_params": {
+ "output_path": "Experiment Settings",
+ "query_path": "/attribute/M:=RNASeq:Replicate/RID={rid}/(Experiment_RID)=(RNASeq:Experiment_Settings:Experiment_RID)/RID,Experiment_RID,Alignment_Format,Aligner,Aligner_Version,Reference_Genome,Sequence_Trimming,Duplicate_Removal,Pre-alignment_Sequence_Removal,Junction_Reads,Library_Type,Protocol_Reference,Library_Selection,Quantification_Format,Quantification_Software,Expression_Metric,Transcriptome_Model,Sequencing_Platform,Paired_End,Read_Length,Strandedness,Used_Spike_Ins,Spike_Ins_Amount,Visualization_Format,Visualization_Software,Visualization_Version,Visualization_Setting,Notes,RCT,RMT?limit=none"
+ }
+ },
+ {
+ "processor": "csv",
+ "processor_params": {
+ "output_path": "Replicate",
+ "query_path": "/attribute/M:=RNASeq:Replicate/RID={rid}/RID,Study_RID,Experiment_RID,Biological_Replicate_Number,Technical_Replicate_Number,Specimen_RID,Collection_Date,Mapped_Reads,GEO_Sample_Accession_ID,Notes,Principal_Investigator,Consortium,Release_Date,RCT,RMT?limit=none"
+ }
+ },
+ {
+ "processor": "csv",
+ "processor_params": {
+ "output_path": "Specimen",
+ "query_path": "/attribute/M:=RNASeq:Replicate/RID={rid}/S:=(Specimen_RID)=(Gene_Expression:Specimen:RID)/T:=left(Stage_ID)=(Vocabulary:Developmental_Stage:ID)/$S/RID,Title,Species,Stage_ID,Stage_Name:=T:Name,Stage_Detail,Assay_Type,Strain,Wild_Type,Sex,Passage,Phenotype,Cell_Line,Parent_Specimen,Upload_Notes,Preparation,Fixation,Embedding,Internal_ID,Principal_Investigator,Consortium,Release_Date,RCT,RMT,GUDMAP2_Accession_ID?limit=none"
+ }
+ },
+ {
+ "processor": "csv",
+ "processor_params": {
+ "output_path": "Specimen_Anatomical_Source",
+ "query_path": "/attribute/M:=RNASeq:Replicate/RID={rid}/(Specimen_RID)=(Gene_Expression:Specimen:RID)/(RID)=(Gene_Expression:Specimen_Tissue:Specimen_RID)/RID,Specimen_RID,Tissue,RCT,RMT?limit=none"
+ }
+ },
+ {
+ "processor": "csv",
+ "processor_params": {
+ "output_path": "Specimen_Cell_Types",
+ "query_path": "/attribute/M:=RNASeq:Replicate/RID={rid}/(Specimen_RID)=(Gene_Expression:Specimen:RID)/(RID)=(Gene_Expression:Specimen_Cell_Type:Specimen)/RID,Specimen_RID:=Specimen,Cell_Type,RCT,RMT?limit=none"
+ }
+ },
+ {
+ "processor": "csv",
+ "processor_params": {
+ "output_path": "Single Cell Metrics",
+ "query_path": "/attribute/M:=RNASeq:Replicate/RID={rid}/(RID)=(RNASeq:Single_Cell_Metrics:Replicate_RID)/RID,Study_RID,Experiment_RID,Replicate_RID,Reads_%28Millions%29,Reads%2FCell,Detected_Gene_Count,Genes%2FCell,UMI%2FCell,Estimated_Cell_Count,Principal_Investigator,Consortium,Release_Date,RCT,RMT?limit=none"
+ }
+ },
+ {
+ "processor": "csv",
+ "processor_params": {
+ "output_path": "File",
+ "query_path": "/attribute/M:=RNASeq:Replicate/RID={rid}/(RID)=(RNASeq:File:Replicate_RID)/RID,Study_RID,Experiment_RID,Replicate_RID,Caption,File_Type,File_Name,URI,File_size,MD5,GEO_Archival_URL,dbGaP_Accession_ID,Processed,Notes,Principal_Investigator,Consortium,Release_Date,RCT,RMT,Legacy_File_RID,GUDMAP_NGF_OID,GUDMAP_NGS_OID?limit=none"
+ }
+ },
+ {
+ "processor": "fetch",
+ "processor_params": {
+ "output_path": "assets/Study/{Study_RID}/Experiment/{Experiment_RID}/Replicate/{Replicate_RID}",
+ "query_path": "/attribute/M:=RNASeq:Replicate/RID={rid}/(RID)=(RNASeq:File:Replicate_RID)/File_Type=FastQ/File_Name::ciregexp::%5B_.%5DR%5B12%5D%5C.fastq%5C.gz/url:=URI,length:=File_size,filename:=File_Name,md5:=MD5,Study_RID,Experiment_RID,Replicate_RID?limit=none"
+ }
+ }
+ ]
+ }
+}
diff --git a/workflow/conf/bdbag.json b/workflow/conf/bdbag.json
new file mode 100644
index 0000000..2c2ab24
--- /dev/null
+++ b/workflow/conf/bdbag.json
@@ -0,0 +1,28 @@
+{
+ "fetch_config": {
+ "http": {
+ "http_cookies": {
+ "file_names": [
+ "*cookies.txt"
+ ],
+ "scan_for_cookie_files": true,
+ "search_paths": [
+ "."
+ ],
+ "search_paths_filter": "*cookies.txt"
+ }
+ },
+ "https": {
+ "http_cookies": {
+ "file_names": [
+ "*cookies.txt"
+ ],
+ "scan_for_cookie_files": true,
+ "search_paths": [
+ "."
+ ],
+ "search_paths_filter": "*cookies.txt"
+ }
+ }
+ }
+}
diff --git a/workflow/conf/multiqc_config.yaml b/workflow/conf/multiqc_config.yaml
new file mode 100644
index 0000000..ed1375a
--- /dev/null
+++ b/workflow/conf/multiqc_config.yaml
@@ -0,0 +1,180 @@
+custom_logo: './bicf_logo.png'
+custom_logo_url: 'https/utsouthwestern.edu/labs/bioinformatics/'
+custom_logo_title: 'Bioinformatics Core Facility'
+
+report_header_info:
+ - Contact Email: 'bicf@utsouthwestern.edu'
+ - Application Type: 'RNA-Seq Analytic Pipeline for GUDMAP/RBK'
+ - Department: 'Bioinformatic Core Facility, Department of Bioinformatics, University of Texas Southwestern Medical Center'
+
+title: RNA-Seq Analytic Pipeline for GUDMAP/RBK
+
+report_comment: >
+ This report has been generated by the <a href="https://doi.org/10.5281/zenodo.3625056">GUDMAP/RBK RNA-Seq Pipeline</a>
+
+top_modules:
+ - fastqc:
+ name: 'Raw'
+ info: 'Replicate Raw fastq QC Results'
+ - cutadapt:
+ name: 'Trim'
+ info: 'Replicate Trim Adapter QC Results'
+ - hisat2:
+ name: 'Align'
+ info: 'Replicate Alignment QC Results'
+ path_filters:
+ - '*alignSummary*'
+ - picard:
+ name: 'Dedup'
+ info: 'Replicate Alignement Deduplication QC Results'
+ - rseqc:
+ name: 'Inner Distance'
+ info: 'Replicate Paired End Inner Distance Distribution Results'
+ path_filters:
+ - '*insertSize*'
+ - custom_content
+ - featureCounts:
+ name: 'Count'
+ info: 'Replicate Feature Count QC Results'
+ - hisat2:
+ name: 'Inference: Align'
+ info: 'Inference Alignment (1M downsampled reads) QC Results'
+ path_filters:
+ - '*alignSampleSummary*'
+ - rseqc:
+ name: 'Inference: Stranded'
+ info: '1M Downsampled Reads Strandedness Inference Results'
+ path_filters:
+ - '*infer_experiment*'
+
+report_section_order:
+ run:
+ order: 4000
+ rid:
+ order: 3000
+ meta:
+ order: 2000
+ ref:
+ order: 1000
+ software_versions:
+ order: -1000
+ software_references:
+ order: -2000
+
+skip_generalstats: true
+
+custom_data:
+ run:
+ file_format: 'tsv'
+ section_name: 'Run'
+ description: 'This is the run information'
+ plot_type: 'table'
+ pconfig:
+ id: 'run'
+ scale: false
+ format: '{}'
+ headers:
+ Session:
+ description: ''
+ Session ID:
+ description: 'Nextflow session ID'
+ Pipeline Version:
+ description: 'BICF pipeline version'
+ Input:
+ description: 'Input overrides'
+ rid:
+ file_format: 'tsv'
+ section_name: 'RID'
+ description: 'This is the identifying RIDs'
+ plot_type: 'table'
+ pconfig:
+ id: 'rid'
+ scale: false
+ format: '{}'
+ headers:
+ Replicate:
+ description: ''
+ Replicate RID:
+ description: 'Replicate RID'
+ Experiment RID:
+ description: 'Experiment RID'
+ Study RID:
+ description: 'Study RID'
+ meta:
+ file_format: 'tsv'
+ section_name: 'Metadata'
+ description: 'This is the comparison of infered metadata, submitter provided, and calculated'
+ plot_type: 'table'
+ pconfig:
+ id: 'meta'
+ scale: false
+ format: '{:,.0f}'
+ headers:
+ Source:
+ description: 'Metadata source'
+ Species:
+ description: 'Species'
+ Ends:
+ description: 'Single or paired end sequencing'
+ Stranded:
+ description: 'Stranded (forward/reverse) or unstranded library prep'
+ Spike-in:
+ description: 'ERCC spike in'
+ Raw Reads:
+ description: 'Number of reads of the sequencer'
+ Assigned Reads:
+ description: 'Final reads after fintering'
+ Median Read Length:
+ description: 'Average read length'
+ Median TIN:
+ description: 'Average transcript integrity number'
+
+ ref:
+ file_format: 'tsv'
+ section_name: 'Reference'
+ description: 'This is the reference version information'
+ plot_type: 'table'
+ pconfig:
+ id: 'ref'
+ scale: false
+ format: '{}'
+ headers:
+ Species:
+ description: 'Reference species'
+ Genome Reference Consortium Build:
+ description: 'Reference source build'
+ Genome Reference Consortium Patch:
+ description: 'Reference source patch version'
+ GENCODE Annotation Release:
+ description: 'Annotation release version'
+ tin:
+ file_format: 'tsv'
+ section_name: 'TIN'
+ description: 'This is the distribution of TIN values calculated by the tool RSeQC'
+ plot_type: 'bargraph'
+ pconfig:
+ id: 'tin'
+ headers:
+ chrom
+ 1 - 10
+ 11 - 20
+ 21 - 30
+ 31 - 40
+ 41 - 50
+ 51 - 60
+ 61 - 70
+ 71 - 80
+ 81 - 90
+ 91 - 100
+
+sp:
+ run:
+ fn: "run.tsv"
+ rid:
+ fn: 'rid.tsv'
+ meta:
+ fn: 'metadata.tsv'
+ ref:
+ fn: 'reference.tsv'
+ tin:
+ fn: '*_tin.hist.tsv'
diff --git a/workflow/nextflow.config b/workflow/nextflow.config
new file mode 120000
index 0000000..2984cee
--- /dev/null
+++ b/workflow/nextflow.config
@@ -0,0 +1 @@
+../nextflow.config
\ No newline at end of file
diff --git a/workflow/nextflowConf b/workflow/nextflowConf
new file mode 120000
index 0000000..8e5c4cf
--- /dev/null
+++ b/workflow/nextflowConf
@@ -0,0 +1 @@
+../nextflowConf/
\ No newline at end of file
diff --git a/workflow/rna-seq.nf b/workflow/rna-seq.nf
new file mode 120000
index 0000000..e5aa8d1
--- /dev/null
+++ b/workflow/rna-seq.nf
@@ -0,0 +1 @@
+../rna-seq.nf
\ No newline at end of file
--
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