diff --git a/excluded_regions_processing.sh b/excluded_regions_processing.sh
index 5fc3582c0ff597279f7e001eb377ec860f3d118c..2f699dcc643a3bada04cc036230ac232e7432517 100644
--- a/excluded_regions_processing.sh
+++ b/excluded_regions_processing.sh
@@ -1,4 +1,7 @@
 # Make a universe of excluded regions of the genome
+cd MakeGencodeTSS
+./make_TSS_file_from_annotation_with_confidence_better.sh ../gencode.v19.annotation.gtf ../biotypes.txt
+gtf2bed  < MakeGencodeTSS/gencode.v19.annotation_capped_sites.gff > gencode.v19.annotation_capped_sites.bed
 
 ## Gencode Protein Coding genes
 bedtools slop -i gencode.v19.annotation_capped_sites.bed -g hg19.chrom.sizes -b 3000 | cut -f1,2,3 > gencode_tss_3kb_flanking.bed