# # metadata for the example astrocyte ChipSeq workflow package # # ----------------------------------------------------------------------------- # BASIC INFORMATION # ----------------------------------------------------------------------------- # A unique identifier for the workflow package, text/underscores only name: 'astrocyte_example' # Who wrote this? author: 'David Trudgian, Peng Lian' # A contact email address for questions email: 'biohpc-help@utsouthwestern.edu' # A more informative title for the workflow package title: 'Astrocyte Example ChIPSeq Workflow' # A summary of the workflow package in plain text description: | This is an example workflow package for the BioHPC astrocyte workflow system. It implements a simple ChIPSeq analysis workflow using BWA and MACS, plus a simple R Shiny visualization application. Please note that start from publish version 0.0.8 and test version 0.0.1, this workflow will only support Astrocyte 0.4.1 and above. # The minimum Astrocyte version that requires to run this workflow. For old pipelines, which do not have this label # a default value of 0.3.1 will be assigned automatically. A request of minimum version less than 0.4.0 will be ignored. minimum_astrocyte_version: '0.4.1' # The Nextflow version that requires to run this workflow. For old pipelines, which do not have this label # a default value of 0.31.0 will be assigned automatically. Please make sure the requested nextflow version is available # in the module list. nextflow_version: '21.04.1' # (Optional) The Nextflow config file to use for this workflow. If provided, the file should exist in workflow/configs nextflow_config: 'biohpc.config' # The container to use for this workflow, none/singularity. If omitted, the default value 'none' will be used. container: 'none' # The version of singularity to use. This is required if container == 'singularity' singularity_version: '3.5.3' # ----------------------------------------------------------------------------- # DOCUMENTATION # ----------------------------------------------------------------------------- # A list of documentation file in .md format that should be viewable from the # web interface. These files are in the 'docs' subdirectory. The first file # listed will be used as a documentation index and is index.md by convention # To supply a title for documentation use a pair of [ 'filename', 'title' ] documentation_files: - [ 'index.md', 'ChIPSeq Example' ] # ----------------------------------------------------------------------------- # NEXTFLOW WORKFLOW CONFIGURATION # ----------------------------------------------------------------------------- # Remember - The workflow file is always named 'workflow/main.f' # The workflow must publish all final output into $baseDir # A list of clueter environment modules that this workflow requires to run. # Specify versioned module names to ensure reproducability. workflow_modules: - 'BWA/0.7.5' - 'picard/1.127' - 'macs/1.4.2' # A list of container images requires to run this workflow. # Specify full path and version names to ensure reproducability. # This keyword is required when 'container' is specified in Astrocyte 0.4.1 and above #workflow_containers: # - # A list of parameters used by the workflow, defining how to present them, # options etc in the web interface. For each parameter: # # REQUIRED INFORMATION # id: The name of the parameter in the NEXTFLOW workflow # type: The type of the parameter, one of: # string - A free-format string # integer - An integer # real - A real number # file - A single file from user data # files - One or more files from user data # select - A selection from a list of values # multiselect - One or more selections from a list of values # # required: true/false, must the parameter be entered/chosen? # description: A user friendly description of the meaning of the parameter # # OPTIONAL INFORMATION # default: A default value for the parameter (optional) # min: Minium value/characters/files for number/string/files types # max: Maxumum value/characters/files for number/string/files types # regex: A regular expression that describes valid entries / filenames # # SELECT TYPE # choices: A set of choices presented to the user for the parameter. # Each choice is a pair of value and description, e.g. # # choices: # - [ 'myval', 'The first option'] # - [ 'myval', 'The second option'] # # NOTE - All parameters are passed to NEXTFLOW as strings... but they # are validated by astrocyte using the information provided above workflow_parameters: - id: fastq type: files required: true description: | One or more input FASTQ files from a ChIPSeq experiment regex: ".*(fastq|fq)" min: 1 - id: index type: select choices: - [ '/project/apps_database/iGenomes/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/genome.fa', 'UCSC hg38'] - [ '/project/apps_database/iGenomes/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/genome.fa', 'UCSC hg19'] - [ '/project/apps_database/iGenomes/Homo_sapiens/UCSC/hg18/Sequence/BWAIndex/genome.fa', 'UCSC hg18'] - [ '/project/apps_database/iGenomes/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/genome.fa', 'UCSC mm10'] - [ '/project/apps_database/iGenomes/Mus_musculus/UCSC/mm9/Sequence/BWAIndex/genome.fa', 'UCSC mm9'] required: true description: | Reference genome for BWA alignment # ----------------------------------------------------------------------------- # SHINY APP CONFIGURATION # ----------------------------------------------------------------------------- # Remember - The vizapp is always 'vizapp/server.R' 'vizapp/ui.R' # The workflow must publish all final output into $baseDir # Name of the R module that the vizapp will run against vizapp_r_module: 'R/3.4.1-gccmkl' # List of any CRAN packages, not provided by the modules, that must be made # available to the vizapp vizapp_cran_packages: - shiny - shinyFiles # List of any Bioconductor packages, not provided by the modules, that must be made # available to the vizapp vizapp_bioc_packages: - # List of any packages to install from GitHub using devtools, that must be # made availavle to the vizapp vizapp_github_packages: -