diff --git a/r.scripts/sc-TissueMapper_RUN.D17.R b/r.scripts/sc-TissueMapper_RUN.D17.R
index 31fad995a2090f2275d19841c752d56325b62a6a..f050771749acadb4b2c1330308c558eb9ad56b4a 100644
--- a/r.scripts/sc-TissueMapper_RUN.D17.R
+++ b/r.scripts/sc-TissueMapper_RUN.D17.R
@@ -98,7 +98,7 @@ option_list=list(
   make_option("--stg",action="store",default="go",type='character',help="Geneset to use for stress ID"),
   #make_option("--hpc.poststress",action="store",default=0.85,type='numeric',help="Max variance cutoff for PCs to use, post-stress"),
   make_option("--res.poststress",action="store",default=0.2,type='numeric',help="Resolution to cluster, post-stress"),
-  make_option("--ds",action="store",default=10000,type='integer',help="Number of cells to downsample"),
+  make_option("--ds",action="store",default=50000,type='integer',help="Number of cells to downsample"),
   #make_option("--hpc.epi",action="store",default=0.85,type='numeric',help="Max variance cutoff for PCs to use, Epi"),
   make_option("--res.epi",action="store",default=0.2,type='numeric',help="Resolution to cluster, Epi"),
   #make_option("--hpc.st",action="store",default=0.85,type='numeric',help="Max variance cutoff for PCs to use, St"),