From 6bc9af29f17506b1a5baf4c5883fc1a9c8e2f7bc Mon Sep 17 00:00:00 2001 From: "Gervaise H. Henry" <gervaise.henry@utsouthwestern.edu> Date: Thu, 15 Nov 2018 14:56:45 -0600 Subject: [PATCH] Update README.md --- README.md | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/README.md b/README.md index 2612d2f..4e297d3 100755 --- a/README.md +++ b/README.md @@ -12,7 +12,9 @@ Determining cellular heterogeneity in the human prostate with single-cell RNA se * PI Email: [douglas.strand@utsouthwestern.edu](mailto:douglas.strand@utsouthwestern.edu) * **ANALYZED DATA FOR QUERYING AT: [StrandLab.net](http://strandlab.net/analysis.php)** * **Raw data at: [GEO](https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE120716) & [GenitoUrinary Development Molecular Anatomy Project (GUDMAP)]("https://doi.org/10.25548/W-R8CM")** -* **Publication at: [BioRxiv](https://www.biorxiv.org/content/early/2018/10/15/439935)** +* **Publication at:** + * Cell Reports: PENDING + * [BioRxiv](https://www.biorxiv.org/content/early/2018/10/15/439935) Data Analysis ------------- @@ -53,7 +55,7 @@ Data Analysis * 4 Run on several downsamples from 1 sample from 1st patient * run bash script [sc\_TissueMapper\-DS\_D17.sh](https://git.biohpc.swmed.edu/StrandLab/sc-TissueMapper_Pr/blob/master/bash.scripts/sc_TissueMapper-DS_D17.sh) * 5 Aggregate and compare several downsamples from #4 - * run bash script [sc\_TissueMapper\_RUN.DS\_D17.aggr.R](https://git.biohpc.swmed.edu/StrandLab/sc-TissueMapper_Pr/blob/master/r.scripts/sc_TissueMapper_RUN.DS_D17.aggr.R) + * run r script [sc\_TissueMapper\_RUN.DS\_D17.aggr.R](https://git.biohpc.swmed.edu/StrandLab/sc-TissueMapper_Pr/blob/master/r.scripts/sc_TissueMapper_RUN.DS_D17.aggr.R) * **Pipeline:** * Link cellranger count/aggr output to analysis * Create demultiplex file to add custom sample groups @@ -88,8 +90,6 @@ Data Analysis * Lineage: * "DEG\_Epi_5FC.txt" DWS generated DEGs of epithelia from FACS population (bulk) RNA-sequencing * "DEG\_FMSt_5FC.txt" DWS generated DEGs of fibromuscular stroma from FACS population (bulk) RNA-sequencing - * "genes.deg.Epi.csv" DWS generated DEGs of epithelial cells from scRNA-Seq of 3 patient aggregate - * "genes.deg.St.csv" DWS generated DEGs of stromal cells from scRNA-Seq of 3 patient aggregate * Epithelia: * "DEG\_BE_5FC.txt" DWS generated DEGs of basal epithelia from FACS population (bulk) RNA-sequencing * "DEG\_LE_5FC.txt" DWS generated DEGs of luminal epithelia from FACS population (bulk) RNA-sequencing -- GitLab