diff --git a/r.scripts/sc-TissueMapper_RUN.D17_FACS.R b/r.scripts/sc-TissueMapper_RUN.D17_FACS.R index c991ebae0218f118363fc41d5031d2f2906a2836..88b96c58b9107c094f6e77143445209d017da856 100644 --- a/r.scripts/sc-TissueMapper_RUN.D17_FACS.R +++ b/r.scripts/sc-TissueMapper_RUN.D17_FACS.R @@ -187,6 +187,7 @@ results.clust.Lin.id <- results[[3]] rm(results) rm(gene.set) +sc10x <- SetAllIdent(object=sc10x,id="Lin") sc10x.Epi <- scSubset(sc10x,i="Lin",g="Epi") if (any(levels(sc10x@ident)=="Unknown")){ sc10x.St <- scSubset(sc10x,i="Lin",g=c("St","Unknown")) @@ -215,6 +216,21 @@ plot(plot) dev.off() rm(plot) +sc10x.St <- RunTSNE(object=sc10x.St,reduction.use="pca",dims.use=1:pc.use.poststress,do.fast=TRUE) +postscript(paste0("./analysis/tSNE/st/tSNE_Sample.eps")) +plot <- TSNEPlot(object=sc10x.St,group.by="samples",pt.size=2.5,do.return=TRUE,vector.friendly=FALSE) +plot <- plot+theme(axis.text.x=element_text(size=20),axis.text.y=element_text(size=20),axis.title.x=element_text(size=20),axis.title.y=element_text(size=20),legend.text=element_text(size=20)) +plot <- plot+guides(colour=guide_legend(override.aes=list(size=10))) +plot(plot) +dev.off() +postscript(paste0("./analysis/tSNE/st/tSNE_res",opt$res.poststress,".eps")) +plot <- TSNEPlot(object=sc10x.St,pt.size=5,do.label=TRUE,label.size=10,do.return=TRUE,vector.friendly=FALSE) +plot <- plot+theme(axis.text.x=element_text(size=20),axis.text.y=element_text(size=20),axis.title.x=element_text(size=20),axis.title.y=element_text(size=20),legend.text=element_text(size=20)) +plot <- plot+guides(colour=guide_legend(override.aes=list(size=10))) +plot(plot) +dev.off() +rm(plot) + gene.set1 <- read_delim("./genesets/genes.deg.BE.csv",",",escape_double=FALSE,trim_ws=TRUE,col_names=TRUE) gene.set1 <- gene.set1[1] gene.set1 <- as.list(gene.set1)