Commit 70c2fcd6 authored by Gervaise Henry's avatar Gervaise Henry 🤠
Browse files

Change create singler object to big

parent 484ea5ec
load("./genesets/scDWS.250.rda")
load("./genesets/cibersort.rda")
singler = CreateSinglerObject(GetAssayData(sc10x,assay="RNA"), annot = NULL, "PdPbPc_deep", min.genes = 0,
singler = CreateBigSingleRObject(GetAssayData(sc10x,assay="RNA"), annot = NULL, "PdPbPc_deep", min.genes = 0,
technology = "10X", species = "Human", citation = "",
ref.list = list(scDWS.250), normalize.gene.length = F, variable.genes = "de",
fine.tune = T, do.signatures = T, clusters = NULL, do.main.types = F,
......@@ -94,7 +94,7 @@ for (i in c("epi","fmst","st","leu")){
dev.off()
}
singler.leu = CreateSinglerObject(GetAssayData(sc10x.leu,assay="RNA"), annot = NULL, "PdPbPc_deep", min.genes = 0,
singler.leu = CreateBigSingleRObject(GetAssayData(sc10x.leu,assay="RNA"), annot = NULL, "PdPbPc_deep", min.genes = 0,
technology = "10X", species = "Human", citation = "",
ref.list = list(cibersort), normalize.gene.length = F, variable.genes = "de",
fine.tune = T, do.signatures = T, clusters = NULL, do.main.types = T,
......
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