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BICF
Astrocyte
rnaseq
Commits
9131c8e0
Commit
9131c8e0
authored
Oct 01, 2020
by
Brandi Cantarel
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update with singularity
parent
5288e775
Pipeline
#8178
failed with stage
in 2 seconds
Changes
3
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1
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3 changed files
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16 additions
and
17 deletions
+16
-17
nextflow.config
nextflow.config
+12
-9
workflow/main.nf
workflow/main.nf
+3
-7
workflow/process_scripts
workflow/process_scripts
+1
-1
No files found.
nextflow.config
View file @
9131c8e0
...
...
@@ -6,22 +6,25 @@ process {
clusterOptions
=
'--hold --no-kill'
queue
=
'128GB,256GB,256GBv1'
withLabel
:
trim
{
container
=
'
goalconsortium/trim_galore:1.0.4
'
container
=
'
trim_galore.sif
'
}
withLabel
:
dnaalign
{
container
=
'
goalconsortium/dna_alignment:1.0.4
'
container
=
'
dna_alignment.sif
'
}
withLabel
:
profiling_qc
{
container
=
'
goalconsortium/profiling_qc:1.0.4
'
container
=
'
profiling_qc.sif
'
}
with
Name
:
starfusion
{
container
=
'
goalconsortium/starfusion:1.0.4
'
with
Label
:
starfusion
{
container
=
'
starfusion.sif
'
}
with
Name
:
ralign
{
container
=
'
goalconsortium/rna_alignment:1.0.4
'
with
Label
:
ralign
{
container
=
'
rna_alignment.sif
'
}
withName
:
geneabund
{
container
=
'goalconsortium/rna_gene_abundance:1.0.4'
withLabel
:
geneabund
{
container
=
'rna_gene_abundance.sif'
}
withLabel
:
rnaseqstat
{
container
=
'rnaseq_dea.sif'
}
}
...
...
workflow/main.nf
View file @
9131c8e0
...
...
@@ -34,23 +34,18 @@ index_path = file(params.genome)
process checkdesignfile {
queue 'super'
module 'parallel/20150122:pigz/2.4
'
label 'trim
'
publishDir "$params.output", mode: 'copy'
input:
file design_file name 'design.ori.txt'
file ("*") from fqs.collect()
output:
file("design.valid.txt") into newdesign
file("*.fastq.gz") into fastqs mode flatten
stdout spltnames
script:
"""
bash $baseDir/scripts/check_inputfiles.sh
perl -p -e 's/\\r\\n*/\\n/g' design.ori.txt > design.fix.txt
perl $baseDir/scripts/check_designfile.pl ${params.pairs} design.fix.txt
bash $repoDir/process_scripts/design_file/checkdesignfile.sh ${params.pairs} design.ori.txt
"""
}
...
...
@@ -159,6 +154,7 @@ process geneabund {
process statanal {
errorStrategy 'ignore'
publishDir "$params.output", mode: 'copy'
label 'rnaseqstat'
input:
file count_file from counts.toList()
file count_sum from ctsum.toList()
...
...
process_scripts
@
8706de70
Compare
96bcf788
...
8706de70
Subproject commit
96bcf78802267e8bb1834c06ffb98c5d700273e3
Subproject commit
8706de703b8b5933f89651e5be0312af96cddb7c
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