diff --git a/workflow/tests/test_pool_and_psuedoreplicate.py b/workflow/tests/test_pool_and_psuedoreplicate.py
index f82ead497b034db554bd11e2342df4bb311bd0e6..7d7fd2c93dd6a55a58261ab2fb17432b405fd19a 100644
--- a/workflow/tests/test_pool_and_psuedoreplicate.py
+++ b/workflow/tests/test_pool_and_psuedoreplicate.py
@@ -64,7 +64,6 @@ def test_check_controls_single(design_experiment_3):
 @pytest.mark.unit
 def test_single_rep(design_experiment_2):
     sys.argv.extend(['--design', 'design_experiment_2'])
-    sys.argv.extend(['--paired', 'true'])
     single_rep = pool_and_psuedoreplicate.main()