diff --git a/CHANGELOG.md b/CHANGELOG.md index 42052941a9ea0031bec279ed55c4f32eca68401d..d83c84e00747cc74bee0409647542c860392be8f 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -1,12 +1,13 @@ # v3.0.0 **User Facing** -* +* Add 5GEX-PE, 5GEX-R2, threeprime as library options **Background** *Known Bugs* * Vizapp does not yet work for Astrocyte * Running in CLI: to set --fastq path of file/s needs to be in quotes +* 2020-A references don't work with with cellranger versions < 4.0.0 (bug) # v2.2.0 **User Facing** diff --git a/README.md b/README.md index f221d97744a0fb293e8c20d9dab4f8f57523954b..9542a95a3ed7125a8002585c9e7428a97e2a4ecf 100755 --- a/README.md +++ b/README.md @@ -96,9 +96,12 @@ To Run: * --version (cellranger version) 2.1.1 can only read --kitVersion of two (2) * options: * *'auto'* + * *'3GEX'* * *'3GEXv3'* * *'3GEXv2'* * *'5GEX'* + * *'5GEXPE'* + * *'5GEXR2'* * eg: **--kitVersion '3GEXv3'** * **--version** * cellranger version diff --git a/astrocyte_pkg.yml b/astrocyte_pkg.yml index 119d8c3a67133993bdebe5ee8816673884d00368..74f1ebf8fe8198bf02b619de8e6e18a9da001d7f 100755 --- a/astrocyte_pkg.yml +++ b/astrocyte_pkg.yml @@ -140,9 +140,12 @@ workflow_parameters: default: 'auto' choices: - ['auto', 'Auto Detect'] + - ['3GEX', '3prime GEX Auto (3prime Gene Expression)'] - ['3GEXv3', '3prime GEX v3 (3prime Gene Expression)'] - ['3GEXv2', '3prime GEX v2 (3prime Gene Expression)'] - ['5GEX', '5prime GEX Auto (5prime Gene Expression)'] + - ['5GEXPE', '5prime GEX both R1 and R2 used for alignment (5prime Gene Expression)'] + - ['5GEXR2', '5prime GEX only R2 used for alignment (5prime Gene Expression)'] required: true description: | 10x single cell gene expression chemistry version (only used in cellranger version 3.x). diff --git a/workflow/main.nf b/workflow/main.nf index 532b661c73b0def69e892f45598e401eced5ba26..e9f5afb1e05fa601a4d1f3a533f493ba7df373f0 100755 --- a/workflow/main.nf +++ b/workflow/main.nf @@ -44,7 +44,9 @@ if (params.astrocyte) { } else { params.genomeLocation = '/project/apps_database/cellranger/refdata-cellranger-' } - if (params.kitVersion == "3GEXv1") { + if (params.kitVersion == "3GEX") { + params.chemistryParam ='threeprime' + } else if (params.kitVersion == "3GEXv1") { params.chemistryParam ='SC3Pv1' } else if (params.kitVersion == "3GEXv2") { params.chemistryParam ='SC3Pv2' @@ -52,6 +54,10 @@ if (params.astrocyte) { params.chemistryParam ='SC3Pv3' } else if (params.kitVersion == "5GEX") { params.chemistryParam ='fiveprime' + } else if (params.kitVersion == "5GEXPE") { + params.chemistryParam ='SC5P-PE' + } else if (params.kitVersion == "5GEXR2") { + params.chemistryParam ='SC5P-R2' } else { params.chemistryParam = 'auto' }